LOCUS       sequence-365539-         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Renilla luciferase via a T7 promoter in TXTL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pTXTL-T7max-RLuc
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..933
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
ORIGIN
        1 atgacttcga aagtttatga tccagaacaa aggaaacgga tgataactgg tccgcagtgg
       61 tgggccagat gtaaacaaat gaatgttctt gattcattta ttaattatta tgattcagaa
      121 aaacatgcag aaaatgctgt tattttttta catggtaacg cggcctcttc ttatttatgg
      181 cgacatgttg tgccacatat tgagccagta gcgcggtgta ttataccaga ccttattggt
      241 atgggcaaat caggcaaatc tggtaatggt tcttataggt tacttgatca ttacaaatat
      301 cttactgcat ggtttgaact tcttaattta ccaaagaaga tcatttttgt cggccatgat
      361 tggggtgctt gtttggcatt tcattatagc tatgagcatc aagataagat caaagcaata
      421 gttcacgctg aaagtgtagt agatgtgatt gaatcatggg atgaatggcc tgatattgaa
      481 gaagatattg cgttgatcaa atctgaagaa ggagaaaaaa tggttttgga gaataacttc
      541 ttcgtggaaa ccatgttgcc atcaaaaatc atgagaaagt tagaaccaga agaatttgca
      601 gcatatcttg aaccattcaa agagaaaggt gaagttcgtc gtccaacatt atcatggcct
      661 cgtgaaatcc cgttagtaaa aggtggtaaa cctgacgttg tacaaattgt taggaattat
      721 aatgcttatc tacgtgcaag tgatgattta ccaaaaatgt ttattgaatc ggacccagga
      781 ttcttttcca atgctattgt tgaaggtgcc aagaagtttc ctaatactga atttgtcaaa
      841 gtaaaaggtc ttcatttttc gcaagaagat gcacctgatg aaatgggaaa atatatcaaa
      901 tcgttcgttg agcgagttct caaaaatgaa caa
//
