LOCUS       sequence-36554-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Tournier C, Whitmarsh AJ, Cavanagh J, Barrett T, Davis RJ
  TITLE     The MKK7 gene encodes a group of c-Jun NH2-terminal kinase kinases.
  JOURNAL   Mol Cell Biol. 1999 Feb . 19(2):1569-81.
  PUBMED    9891090
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1999 Feb . 19(2):1569-81."
COMMENT     Sequence Label: pCDNA3 Flag MKK7b1(glu)
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     57..76
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        57..75
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             103..126
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(253..264)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 cgnggtttnc gactctctgg ctactgagaa cccactgctt actggcttat cgaaattaat
       61 acgactcact atagggagac ccaagcttcg gcagccaaca tggactataa ggacgatgat
      121 gacaaaatgg cggcgtcctc cctggagcag aagctgtccc gcctggaagc caagctgaag
      181 caggagaacc gtgaggcccg caggaggatc gacctcaact tggatatcag cccacagcgg
      241 cccaggccca ccctgcaact cccactggcc aacgatgggg gcagccgctc accatcctca
      301 gagagctccc cacagcaccc tacacccccc acccggcccc gccacatgct ggggctccca
      361 tcaaccttgt tcacaccgcg cagtatggag agcatcgaga ttgaccagaa gctgcaggag
      421 atcatgaagc agacagggta cctgactatc gggggccagc gttatcaggc agaaatcaat
      481 gacttggaga acttgggtga gatgggcagt ggtacctgtg gtcaggtgtg gaagatgcgg
      541 ttccggaaga caggccacat cattgctgtt aagcaaatgc ggcgctctgg gaacaaggaa
      601 gagaataagc gcattttgat ggacctggat gtagtactca agagccatga ctgcccttac
      661 atcgttcagt gctttggcac cttcatcacc aacacagacg tctttattgc catggagctc
      721 atgggcacat gtgcagagaa gctgaagaaa cgaatgcagg gccccattcc agagcgaatc
      781 ctgggcaaga tgactgtggc gattgtgaaa gcactgtact atctgaagga gaagcatggc
      841 ttctnntcac ca
//
