LOCUS       sequence-365543-         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of eGFP-Luz via a T7 promoter in TXTL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pTXTL-T7max-HisGFPLuz
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgcgtatca acatctccct gtcgtcgtta ttcgaacggt taagtaaact gagctctcgt
       61 tcaattgcga ttacctgtgg agttgtcctc gcctctgcta ttgcttttcc catcatacgt
      121 cgggattatc agacgttcct ggaggttggt cctagctatg ccccgcaaaa ctttcgcggc
      181 tacattatcg tttgcgtact tagcttgttc cgccaagagc agaaaggcct ggcgatctac
      241 gaccggctcc cagagaagcg ccgctggctg gcggatctgc catttcgtga aggtaccaga
      301 ccgagtatta cctcacacat tatccagcgc cagcgcactc aacttgtgga tcaggaattt
      361 gccacacgtg aactgattga caaggttatt ccgagggtgc aagcacgaca taccgataaa
      421 acatttttga gcacgagcaa gtttgaattt catgcgaaag ccattttcct gttgccatcg
      481 attccaatca acgacccgct gaacatacct agtcatgata cggtgcgtcg aacgaaacgc
      541 gaaattgccc acatgcatga ctatcacgat tgcacccttc acctagctct ggcggcacag
      601 gatggaaaag aagtcctgaa aaaaggctgg ggccagcgtc atccgctggc ggggccgggt
      661 gtgcctgggc cgccgactga atggactttc ttatacgcac cccgtaatga ggaagaagcg
      721 cgcgtggtag aaatgattgt ggaagcatcc atcggctata tgaccaatga tccggcaggt
      781 aaaatcgtcg agaatgccaa a
//
