LOCUS       sequence-36555-e         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Certo MT, Ryu BY, Annis JE, Garibov M, Jarjour J, Rawlings DJ, 
            Scharenberg AM
  TITLE     Tracking genome engineering outcome at individual DNA breakpoints.
  JOURNAL   Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648.
  PUBMED    21743461
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-07-10- 10"; volume: "8"; 
            issue: "8"; pages: "671-6"
COMMENT     Sequence Label: pRRL SFFV d20GFP.T2A.mTagBFP Donor
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..729)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
ORIGIN
        1 tccagaggtt gattgtcgac tctagaatca attaagcttg tgccccagtt tgctagggag
       61 gtcgcagtat ctggccactg ccacctcgtg ctgctcgacg taggtctcgt tgttggcctc
      121 cttgattctt tccagtctgt agtccacata gtagacgcca ggcatcttga ggttcttagc
      181 gggtttcttg gatctatatg tggtcttgat gtttgcgatc agatggctcc cgcccacgag
      241 cttcagggcc atgtcgtttc tgccttccag gccgccgtca gcggggtaca gcgtctcggt
      301 gaaggcctcc cagccgagtg ttttcttctg catcacaggg ccgttggatg tgaagttcac
      361 ccctctgatc ttgacgttgt agatgaggca gccgtcctgg aggctggtgt cctgggtagc
      421 ggtcagcacg cccccgtctt cgtatgtggt gactctctcc catgtgaagc cctcagggaa
      481 ggactgcttg aagaagtcgg ggatgccctg ggtgtggttg atgaaggtct tgctgccgta
      541 gaggaagcta gtagccagga tgtcgaaggc gaaggggaga gggccgccct cgaccacctt
      601 gattctcatg gtctgggtgc cctcgtaggg cttgccttcg ccctcggatg tgcacttgaa
      661 gtgatggttg tccacggtgc cctccatgta cagcttcatg tgcatgttct ccttaatcag
      721 ctcgctcatg gggcccggat tctcctccac gtcaccgcat gttagaagac
//
