LOCUS       sequence-365553-         675 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of HiBiT-GFP via T7 promoter in TXTL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pTXTL-T7max-HiBiTGFPHis
FEATURES             Location/Qualifiers
     source          1..675
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(31..52)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(292..311)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     639..660
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggagcttt tcactggcgt tgttcccatc ctggtcgagc tggacggcga cgtaaacggc
       61 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      121 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
      181 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
      241 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
      301 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      361 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
      421 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
      481 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
      541 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
      601 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
      661 accgccgccg ggatc
//
