LOCUS       sequence-365557-         477 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of LgBiT via sigma 70 promoter in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pLumi-LgBiT
FEATURES             Location/Qualifiers
     source          1..477
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggtcttca cactcgaaga tttcgttggg gactgggaac agacagccgc gtacaacctg
       61 gaccaagtcc ttgaacaggg aggtgtgtcc agtttgctgc agaatctcgc ggtgtccgta
      121 actccgatcc aaaggattgt ccgcagcggt gaaaatgcgc tgaagatcga catccatgtc
      181 atcatcccgt atgaaggtct gagcgcggac caaatggcac agatcgaaga agtgtttaag
      241 gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc tgccgtatgg cacactggta
      301 atcgacgggg ttacgccgaa catgctgaac tatttcggac ggccgtatga aggcatcgcc
      361 gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa caaaattatc
      421 gacgagcgcc tgatcacccc cgacggctcc atgctgttcc gagtaaccat caacagc
//
