LOCUS       sequence-365559-        1184 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of H3H via a T7 promoter in TXTL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pTXTL-T7max-H3H
FEATURES             Location/Qualifiers
     source          1..1184
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             1141..1157
                     /estimated_length=17
ORIGIN
        1 tnnntcnnnn nnnnngtggn gtggtganga tggtgatggc cacctccacg cgtagctgag
       61 ttggatgatc gcaacagtgt ttccaaaacc ttctctgact ctaccttaat atcataaccc
      121 atcactctgt ctctcaattc tttggaccta aggtacagcc ctctcatctc tgggacatat
      181 tgctcctcat agctctctgc ggcgacaaac tcagctcgat cctttcgcag ctgttcgtaa
      241 acggccagcc ttgtctcgat ctgtgatgcg gtcgttccct taggcaacaa cgtgcccaaa
      301 gcgactgcat cctccaaccc catcccgaag ccttgtccaa gtgtgggcag tgaagcatgg
      361 gcggcatcac ccagcaagca taccctcttg ttcacccatt ttttcaagac cgggaccacc
      421 ctcatctgcc ataaattaat gtcctgtgct aactctaaga gtcgtcggaa tcttggatgg
      481 taatcgtgaa acactctgaa taaatcatcc tttgatgcgg gaacggacca atcagcagtg
      541 ttttgatctc tttcgtcgtc gtgtacggct aatacattaa ccatggtccc acctctgcat
      601 gcgtaaataa tgatcgttcg tctatcggcc agtcctacgc cactctgcgg gttctgtgga
      661 gtagaaatca acctggctcc caagggtggt tttttgacaa tccaatccag ttcgggatat
      721 gggtcgagag catcggcctg gaccaaccat cgaaagccga caattccgct agctggaaca
      781 gtcacgtcct cctccagaac gaatcttcga atcgtagacc taataccatc agctccgaca
      841 atcaagtcag cagactgtgt cgtcccgntc gtaaacgtga ctgtacaggc atccacatcg
      901 cagaacgtaa cacgatggtt aanntgaagn agtggncann natggnnnnt aanccccgcn
      961 ccngnatcca aggcgactcg catcagctcn nnnnnggnan nnnccanntc gnttnnncca
     1021 ttnatnccnn naagtnnnna tgcnnccacg gntagntcgg nncnttatta ttncatcatn
     1081 cnnnnnctnc cngtncnnnt nncnntggnc ngnnnannct nnnnnngnnt acnnnnnttc
     1141 nnnnnnnnnn nnnnnnnccn nnnnngnnnn gcnnggnnaa nncg
//
