LOCUS       sequence-36556-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Junio AB, Li X, Massey HC, Nolan TJ, Todd Lamitina S, Sundaram MV, 
            Lok JB
  TITLE     Strongyloides stercoralis: cell- and tissue-specific transgene 
            expression and co-transformation with vector constructs 
            incorporating a common multifunctional 3' UTR.
  JOURNAL   Exp Parasitol. 2008 Feb . 118(2):253-65.
  PUBMED    17945217
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Parasitol. 2008 Feb . 118(2):253-65."
COMMENT     Sequence Label: pAJ13
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(317..333)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(317..333)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(330..352)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    341..357
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(365..371,372..389,390..395))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    410..431
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(548..565)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(799..818)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccantattca cagaattggg acactccagt gaaaagttct tctcctttac tcattttttc
       61 taccggtacc ctccaagggt cctcctgaaa atgttctatg ttatgttagt atcattcgaa
      121 acataccttt gggtcctttg gccaatcccg gggatcctct agagtcgacc tgcaggcatg
      181 caagcttatt tcatttccaa gttgttagcg tatccatcgt tgtgagtgat ttcagccatt
      241 tttaagctct gaaaatttaa atgagaatag ttagttagta agatcatgtt taaacttaca
      301 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
      361 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
      421 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
      481 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
      541 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
      601 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
      661 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
      721 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      781 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
      841 ctctgtccga ctgcntacga cgccttctca ggtca
//
