LOCUS       sequence-365560-        1202 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of H3H via a T7 promoter in TXTL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pTXTL-T7max-H3H
FEATURES             Location/Qualifiers
     source          1..1202
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             2..13
                     /estimated_length=12
     gap             1134..1145
                     /estimated_length=12
     gap             1157..1174
                     /estimated_length=18
ORIGIN
        1 cnnnnnnnnn nnntnagtnn ggcatccttt ganttcactc agtgtcttaa tagttggggc
       61 tggcttaggt ggtttggccg ctgctatagc actgcgaagg caaggacatg tagtcaagat
      121 atacgatagt tcttcattca aggccgaatt gggagcagga ttagctgtac cacccaatac
      181 tctccgttcc ctgcaacaac tgggctgtaa tacagaaaac ctgaacggag tagacaacct
      241 ctgctttacg gccatgggat acgacgggag tgtaggtatg atgaataata tgaccgacta
      301 ccgtgaggca tacggcactt cctggataat ggtacatcga gtggatctcc acaacgagct
      361 gatgcgagtc gccttggatc cgggcgggtt agggcctcct gccactcttc accttaacca
      421 tcgtgttacg ttctgcgatg tggatgcctg tacagtcacg tttacgaacg ggacgacaca
      481 gtctgctgac ttgattgtcg gagctgatgg tattaggtct acgattcgaa gattcgttct
      541 ggaggaggac gtgactgttc cagctagcgg aattgtcggc tttcgatggt tggtccaggc
      601 cgatgctctc gacccatatc ccgaactgga ttggattgtc naaaaaccnc ncttgggagc
      661 caggttgatt tctactccac agaacccgca gagtggcgta ggactggccg atagacgaac
      721 gatcatttat ttacgcattg cagaggtggg accatggtta atgtattagc cgtacacgac
      781 gacnaaagag atcaaaacac tgctgattgg tccgttcccg catctaanga tgatttattc
      841 agagtgtttt nncganttac catccaagat tccgacgacn tcttanagtt agcacaggac
      901 ntttantttn tgggcagatg angggtggtc ccngntcttt gnnnnanatg ggntgaanca
      961 agaaggntat gcntgnntgg ggtgaatgcc gccnnatgct tnnactgccc nncacttngn
     1021 ananngnnnc nnnntgggnn nnnnngtatg cngtcnctnn tgggcnnncg tnngntgncn
     1081 nnannnnnnc nnancnncnn nnncnnnatt nnnnnnnant gnnttnnnng nttnnnnnnn
     1141 nnnnntnnnn nnatggnnnn nnnnnnnnnn nnnncnnnna annnnnnntg naannnnnnn
     1201 nc
//
