LOCUS       sequence-365561-        1056 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Luz via a T7 promoter in TXTL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pTXTL-T7max-Luz
FEATURES             Location/Qualifiers
     source          1..1056
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             2..11
                     /estimated_length=10
     CDS             840..863
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 cnnnnnnnnn ngnnntaann nnngcggnnn caacatctcc ctgtcgtcgt tattcgaacg
       61 gttaagtaaa ctgagctctc gttcaattgc gattacctgt ggagttgtcc tcgcctctgc
      121 tattgctttt cccatcatac gtcgggatta tcagacgttc ctggaggttg gtcctagcta
      181 tgccccgcaa aactttcgcg gctacattat cgtttgcgta cttagcttgt tccgccaaga
      241 gcagaaaggc ctggcgatct acgaccggct cccagagaag cgccgctggc tggcggatct
      301 gccatttcgt gaaggtacca gaccgagtat tacctcacac attatccagc gccagcgcac
      361 tcaacttgtg gatcaggaat ttgccacacg tgaactgatt gacaaggtta ttccgagggt
      421 gcaagcacga cataccgata aaacattttt gagcacgagc aagtttgaat ttcatgcgaa
      481 agccattttc ctgttgccat cgattccaat caacgacccg ctgaacatac ctagtcatga
      541 tacggtgcgt cgaacgaaac gcgaaattgc ccacatgcat gactatcacg attgcaccct
      601 tcacctagct ctggcggcac aggatggaaa agaagtcctg aaaaaaggct ggggccagcg
      661 tcatccgctg gcggggccgg gtgtgcctgg gccgccgact gaatggactt tcttatacgc
      721 accccgtaat gaggaagaag cgcgcgtggt agaaatgatt gtggaagcat ccatcggcta
      781 tatgaccaat gatccggcag gtaaaatcgt cgagaatgcc aaaacgcgtg gnngtggncc
      841 atcaccatca tcaccatcac cacggataac tcgagcaaag cccgcccgaa aggcgggctt
      901 ttctgtannn aacaattgca ttcattttna tgtttcnggt tcagggggnn atgtgggnnt
      961 ttttttaaag caagtaaaan ntctannaan gtggnaaaat cgannnnnnn cnnnntnnng
     1021 tantagcnan angnngcncn atcnnnttnc caacag
//
