LOCUS       sequence-365563-        1087 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of LuxA via a T7 promoter in TXTL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pTXTL-T7max-LuxA
FEATURES             Location/Qualifiers
     source          1..1087
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             1050..1083
                     /estimated_length=34
ORIGIN
        1 agtnngaatt nggaactttt tgcttacata ccaacctccc caattttctc aaacagaggt
       61 aatgaaacgt ttggttaaat taggtcgcat ctctgaggag tgtggttttg ataccgtatg
      121 gttactggag catcatttca cggagtttgg tttgcttggt aacccttatg tcgctgctgc
      181 atatttactt ggcgcgacta aaaaattgaa tgtaggaact gccgctattg ttcttcccac
      241 agcccatcca gtacgccaac ttgaagatgt gaatttattg gatcaaatgt caaaaggacg
      301 atttcggttt ggtatttgcc gagggcttta caacaaggac tttcgcgtat tcggcacaga
      361 tatgaataac agtcgcgcct tagcggaatg ctggtacggg ctgataaaga atggcatgac
      421 agagggatat atggaagctg ataatgaaca tatcaagttc cataaggtaa aagtaaaccc
      481 cgcggcgtat agcagaggtg gcgcaccggt ttatgtggtg gctgaatcag cttcgacgac
      541 tgagtgggct gctcaatttg gcctaccgat gatattaagt tggattataa atactaacga
      601 aaagaaagca caacttgagc tttataatga agtggctcaa gaatatgggc acgatattca
      661 taatatcgac cattgcttat catatataac atctgtagat catgactcaa ttaaagcgaa
      721 agagatttgc cggaaatttc tggggcattg gtatgattct tatgtgaatg ctacgactat
      781 tttttgatga ttcagaccaa acaagaggtt atgatttcaa taaagggcag tggcgtgact
      841 ttgtattaaa aggacataaa gatactaatc gccgtattga ttacagtnac gnnncnatcc
      901 cgtggngaac gccgcnggaa tgtattgaca tnanttcaaa aanacnttgg atgcttacng
      961 nnnntcnann atttgnngng gntttgaagc taatnnnnna nnagacgaaa nnnnngcttn
     1021 nntnnannnt tcnnnncnnn tcngnnntgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnntttc
//
