LOCUS       sequence-365565-        1099 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of LuxB via a T7 promoter in TXTL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pTXTL-T7max-LuxB
FEATURES             Location/Qualifiers
     source          1..1099
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             1052..1066
                     /estimated_length=15
     gap             1078..1087
                     /estimated_length=10
ORIGIN
        1 tagtnngann tggantgttc ttccttaact tcatcaattc aacaactgtt caagaacaaa
       61 gtatagttcg catgcaggaa ataacggagt atgttgataa gttgaatttt gaacagattt
      121 tagtgtatga aaatcatttt tcagataatg gtgttgtcgg cgctcctctg actgtttctg
      181 gttttctgct cggtttaaca gagaaaatta aaattggttc attaaatcac atcattacaa
      241 ctcatcatcc tgtccgcata gcggaggaag cttgcttatt ggatcagtta agtgaaggga
      301 gatttatttt agggtttagt gattgcgaaa aaaaagatga aatgcatttt tttaatcgcc
      361 cggttgaata tcaacagcaa ctatttgaag agtgttatga aatcattaac gatgctttaa
      421 caacaggcta ttgtaatcca gataacgatt tttatagctt ccctaaaata tctgtaaatc
      481 cccatgctta tacgccaggc ggacctcgga aatatgtaac agcaaccagt catcatattg
      541 ttgagtgggc ggccaaaaaa ggtattcctc tcatctttaa gtgggatgat tctaatgatg
      601 ttagatatga atatgctgaa agatataaag ccgttgcgga taaatatgac gttgacctat
      661 cagagataga ccatcagtta atgatattag ttaactataa cgaagatagt aataaagcta
      721 aacaagagac gcgtgcattt attagtgatt atgttcttga aatgcaccct aatgaaaatt
      781 tcgaaaataa acttgaagaa ataattgcag aaaacgctgt cggaaattat acggagtgta
      841 taactgcggc taagttggca attgaaaagt gtggtgcgaa aagtgtattg ctgtcctttg
      901 aaccaatgna ntgatttgat gagcccaaaa aaaaatgtaa tcnatattnn ngnatgannn
      961 nanttaannn naccacatnn nntataccnt anncnnnnaa agcccnncnn annnncgnnn
     1021 tttnntgtan nnnnnatnnc nntnnnnnna tnnnnnnnnn nnnnnngnan nnnnnnannn
     1081 nnnnnnnaaa nnnnnngnt
//
