LOCUS       sequence-365566-        1095 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of LuxB via a T7 promoter in TXTL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pTXTL-T7max-LuxB
FEATURES             Location/Qualifiers
     source          1..1095
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             1083..1093
                     /estimated_length=11
ORIGIN
        1 tcnnntnggn tnttccatgt ggtacttctt aatattatca tcaacaatat tgattacatt
       61 tttttggctc atcaaatcat tcattggttc aaaggacagc aatacacttt tcgcaccaca
      121 cttttcaatt gccaacttag ccgcagttat acactccgta taatttccga cagcgttttc
      181 tgcaattatt tcttcaagtt tattttcgaa attttcatta gggtgcattt caagaacata
      241 atcactaata aatgcacgcg tctcttgttt agctttatta ctatcttcgt tatagttaac
      301 taatatcatt aactgatggt ctatctctga taggtcaacg tcatatttat ccgcaacggc
      361 tttatatctt tcagcatatt catatctaac atcattagaa tcatcccact taaagatgag
      421 aggaatacct tttttggccg cccactcaac aatatgatga ctggttgctg ttacatattt
      481 ccgaggtccg cctggcgtat aagcatgggg atttacagat attttaggga agctataaaa
      541 atcgttatct ggattacaat agcctgttgt taaagcatcg ttaatgattt cataacactc
      601 ttcaaatagt tgctgttgat attcaaccgg gcgattaaaa aaatgcattt catctttttt
      661 ttcgcaatca ctaaacccta aaataaatct cccttcactt aactgatcca ataagcaagc
      721 ttcctccgct atgcggacag gatgatgagt tgtaatgatg tgatttaatg aaccaatttt
      781 aattttctct gttaaaccga gcagaaaacc agaaacagtc agangagcgc cgacaacacc
      841 attatctgaa aaaatgattt ttcatacact aaaatctgtt caaaaatttc aacttnatcn
      901 acatactncn nnnatttccc tgcatgcgaa ctatactttg tnttgnacag ttngnttgnn
      961 tngnatgnan ntnnnnnnnn natcnnnatt catactagtc atancggnnt atctnnnnnt
     1021 aannnaannn nnnnntnnnn nntagngnnn ncnnnttnnn nnnnnannng nnnnngnccn
     1081 ngnnnnnnnn nnngc
//
