LOCUS       sequence-365568-        1077 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of LuxC via a T7 promoter in TXTL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pTXTL-T7max-LuxC
FEATURES             Location/Qualifiers
     source          1..1077
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             982..991
                     /estimated_length=10
     gap             1038..1063
                     /estimated_length=26
ORIGIN
        1 gnnnnnnttg ggnnaatana ggaacttatc ttcttccagg aatcgagtct gttctatttc
       61 aaccgcaaca tccttagccg tatagttaga tggcctttca tgagaaatat atgtcactaa
      121 tcgttgcaac ggtctcattc cgtcatgaga tccaccaact cgaaatatgt tattcattcc
      181 tgcttctaca atcctttccg caccttttaa tgctaacgca tctcgatatt taaatgatga
      241 ctcccaagga aaaatagata tggtttgcgt cttatttttt tgaacataag gcaatatttg
      301 ctcaatatta tcgacgtgat gaaggtacac acatctgcca agtggttgat taaattccac
      361 acctgcattt gactcaataa tcatccaacg ttgatgaata tccacctcta cttttaatcc
      421 agcaaacaag ctttcttttt gaactaaaga ataggccgcc ttttcatcaa aatctttttt
      481 ggcattcggt aatatatgcg catatagatt aagtttttct atcaacgcta acttaaattc
      541 ctcataatga tttcccatgt aatatatgtt ttgggcagaa aaacaagctc gctgatcgta
      601 aaaacaaaca tcatgagccg cacctgtcgc tgcggacgtc aaatcaacag gattatcgat
      661 aatgcaaaga ctctttttag aaccaaattt aatcacatca gcataagatg gcgcatgctc
      721 taccgcccaa ttaatcgcat ctggccctcc ccaagcgaca ataacatccg catgttgcat
      781 aatttctttt gcgagtgntg tatcaccttg gtgggggcca atatataaca gataaagagc
      841 gcgttntcgg ntgattnnng tctacatcaa taaaacttaa cgctaatgca ttancggtaa
      901 aaggnncgnn tgnacgatgt anttnnnaat acnctgantn cnttannnnn aattgcgcgt
      961 nntannnnnn atgnncccag annnnnnnnn ntnnaccnnn nnnanagnnt nnnncngnnn
     1021 tnanntttnn gnaanagnnn nnnnnnnnnn nnnnnnnnnn nnngnnnnna nnnnatt
//
