LOCUS       sequence-365569-        1111 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of LuxD via a T7 promoter in TXTL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pTXTL-T7max-LuxD
FEATURES             Location/Qualifiers
     source          1..1111
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             1075..1084
                     /estimated_length=10
     gap             1089..1107
                     /estimated_length=19
ORIGIN
        1 ccnnnnnnnn ntagtnngga ntgatcnnaa tataaaacca tcgaccacgt tatttgtgtt
       61 gaaggaaata aaaaaattca tgtttgggaa acgctgccag aagaaaacag cccaaagaga
      121 aagaatgcca ttattattgc gtctggtttt gcccgcagga tggatcattt tgctggtctg
      181 gcggaatatt tatcgcggaa tggatttcat gtgatccgct atgattcgct tcaccacgtt
      241 ggattgagtt cagggacaat tgatgaattt acaatgtcta taggaaagca gagcttgtta
      301 gcagtggttg attggttaac tacacgaaaa ataaataact tcggtatgtt ggcttcaagc
      361 ttatctgcgc ggatagctta tgcaagccta tctgaaatca atgcttcgtt tttaatcacc
      421 gcagtcggtg ttgttaactt aagatattct cttgaaagag ctttagggtt tgattatctc
      481 agtctaccca ttaatgaatt gccgaataat ctagattttg aaggccataa attgggtgct
      541 gaagtctttg cgagagattg tcttgatttt ggttgggaag atttagcttc tacaattaat
      601 aacatgatgt atcttgatat accgtttatt gcttttactg caaataacga taattgggtc
      661 aagcaagatg aagttatcac attgttatca aatattcgta gtaatcgatg caagatatat
      721 tctttgttag gaagttcgca tgacttgagt gaaaatttag tggtcctgcg caatttttat
      781 caatcggtta cgaaagccgc tatcgcgatg gataatgatc atctggatat tgatgttgat
      841 attactgaac cgtcatttga acatttaact attgcgacag tcaatgaacg cccgaatgag
      901 aanttgagat tgaaaatcaa gcaatttctc tgtcnttaac tcgagcaang ccccgccgaa
      961 nngcggggnt tttnntgtac nnnaccantg ncattncatt nnntgtttnc nnnnnnnggg
     1021 ggnnnatgnn nngnnnnttn nnncnntann nntacnangt gnaaancgnn nnnannnnnn
     1081 nnnntanann nnnnnnnnnn nnnnnnnant c
//
