LOCUS       sequence-365570-        1113 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of LuxD via a T7 promoter in TXTL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pTXTL-T7max-LuxD
FEATURES             Location/Qualifiers
     source          1..1113
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             1053..1063
                     /estimated_length=11
     gap             1075..1087
                     /estimated_length=13
     gap             1100..1109
                     /estimated_length=10
ORIGIN
        1 ctcgnntnag anaganaatt gcttgatttt caatctcaat tctcattcgg cgttcattga
       61 ctgtcgcaat agttaaatgt tcaaatgacg gttcagtaat atcaacatca atatccagat
      121 gatcattatc catcgcgata gcggctttcg taaccgattg ataaaaattg cgcaggacca
      181 ctaaattttc actcaagtca tgcgaacttc ctaacaaaga atatatcttg catcgattac
      241 tacgaatatt tgataacaat gtgataactt catcttgctt gacccaatta tcgttatttg
      301 cagtaaaagc aataaacggt atatcaagat acatcatgtt attaattgta gaagctaaat
      361 cttcccaacc aaaatcaaga caatctctcg caaagacttc agcacccaat ttatggcctt
      421 caaaatctag attattcggc aattcattaa tgggtagact gagataatca aaccctaaag
      481 ctctttcaag agaatatctt aagttaacaa caccgactgc ggtgattaaa aacgaagcat
      541 tgatttcaga taggcttgca taagctatcc gcgcagataa gcttgaagcc aacataccga
      601 agttatttat ttttcgtgta gttaaccaat caaccactgc taacaagctc tgctttccta
      661 tagacattgt aaattcatca attgtccctg aactcaatcc aacgtggtga agcgaatcat
      721 agcggatcac atgaaatcca ttccgcgata aatattccgc cagaccagca aaatgatcca
      781 tcctgcgggc aaaaccagac gcaataataa tggcattctt tctctttggg ctgttttctt
      841 ctggcagcgt ttcccaaaca tgaatttttt tttatttcct tcaacacaaa taacgtgggt
      901 cgatgggttt tatatttttg attcattttt ccatactagt cataccgggt atatctncct
      961 tcnttaaann tnaaacaaaa ttatttccct atantgagtc gnnnntanaa atttanatnc
     1021 tngtcgannn atgnnnncaa annnnnagcn aannnnnnnn nnngnnaaaa anggnnnnnn
     1081 nnnnnnncnn ntnnnnnntn nnnnnnnnnc ccc
//
