LOCUS       sequence-365571-        1083 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of LuxE via a T7 promoter in TXTL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pTXTL-T7max-LuxE
FEATURES             Location/Qualifiers
     source          1..1083
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             1047..1074
                     /estimated_length=28
ORIGIN
        1 tnnnnnnant nngncttcnt atgttgnnaa acaagaaatt acagcaagct cagaaattga
       61 tgatttgatt ttttcgagcg atccattagt gtggtcttac gacgagcagg aaaaaatcag
      121 aaagaaactt gtgcttgatg catttcgtaa tcattataaa cattgtcgag aatatcgtca
      181 ctactgtcag gcacacaaag tagatgacaa tattacggaa attgatgaca tacctgtatt
      241 cccaacatcg gtttttaagt ttactcgctt attaacttct caggaaaacg agattgaaag
      301 ttggtttacc agtagcggca cgaatggttt aaaaagtcag gtggcgcgtg acagattaag
      361 tattgagaga ctcttaggct ctgtgagtta tggcatgaaa tatgttggta gttggtttga
      421 tcatcaaata gaattagtca atttgggacc agatagattt aatgctcata atatttggtt
      481 taaatatgtt atgagtttgg tggaattgtt atatcctacg acatttaccg taacagaaga
      541 acgaatagat tttgttaaaa cattgaatag tcttgaacga ataaaaaatc aagggaaaga
      601 tctttgtctt attggttcgc catactttat ttatttactc tgccattata tgaaagataa
      661 aaaaatctca ttttctggag ataaaagcct ttatatcata accggaggcg gctggaaaag
      721 ttacgaaaaa gaatctctga aacgtgatga tttcaatcat cttttatttg atactttcaa
      781 tctcagtgat attagtcaga tccgagatat atttaatcaa gttgaactca acacttgttt
      841 ctttgaggat gaaatgcagc gtaaacatgt tcccgcccgt gggtatatgc gcgagcgctt
      901 ngatcctgaa acgttgaaan cctgtacctg atgggnnncc gggnntngat gagttatann
      961 ggntgncgtc agcnnnnnnn nntnccnanc ntttantnnn nncngantgg natgtcgnnn
     1021 tnnnnnncna nanaatannn naagnannnn nnnnnnnnnn nnnnnnnnnn nnnncnnnnn
     1081 nnc
//
