LOCUS       sequence-365572-        1082 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of LuxE via a T7 promoter in TXTL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pTXTL-T7max-LuxE
FEATURES             Location/Qualifiers
     source          1..1082
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             1055..1067
                     /estimated_length=13
ORIGIN
        1 cnnntnctat caaacgcttc ggttaagctt aaagcacacc ctttctgcgt cctcgtattg
       61 acgcgacgta aaatttcaac gagcacgccg ggatacttac catattctct gctaattatc
      121 ccgacatcat cggtaacaat aaatgctgga taactggttg ctgacgcatc catataactc
      181 atcaaccccg gcgttccatc aggtacaggt ttcaacgttt caggatcaag cgctcgcgca
      241 tatacccacg gcggaacatg tttacgctgc atttcatcct caaagaaaca agtgttgagt
      301 tcaacttgat taaatatatc tcggatctga ctaatatcac tgagattgaa agtatcaaat
      361 aaaagatgat tgaaatcatc acgtttcaga gattcttttt cgtaactttt ccagccgcct
      421 ccggttatga tataaaggct tttatctcca gaaaatgaga tttttttatc tttcatataa
      481 tggcagagta aataaataaa gtatggcgaa ccaataagac aaagatcttt cccttgattt
      541 tttattcgtt caagactatt caatgtttta acaaaatcta ttcgttcttc tgttacggta
      601 aatgtcgtag gatataacaa ttccaccaaa ctcataacat atttaaacca aatattatga
      661 gcattaaatc tatctggtcc caaattgact aattctattt gatgatcaaa ccaactacca
      721 acatatttca tgccataact cacagagcct aagagtctct caatacttaa tctgtcacgc
      781 gccacctgac ttttttaaac cattcgtgcc gctactggta aaccaacttt caatctcgtt
      841 ttcctgagaa gttaataagc gagtaaactt aaaaaccgat gttgggaata caggtatgtc
      901 atcaatttcc gtaatattgt catctacttt gngtgcctga cagtanngnc gatnntntcg
      961 annntgnttn nnanngnntt ancgaaatgc atcnangcnn nnnnnntttn ngantttnnn
     1021 ccnngcnncn ntcnnnnnan nncacnnnnn nntcnnnnnn nnnnnnnanc ancannnnnn
     1081 ga
//
