LOCUS       sequence-365573-        1110 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Fre via a T7 promoter in TXTL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pTXTL-T7max-Fre
FEATURES             Location/Qualifiers
     source          1..1110
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             720..743
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     gap             1037..1051
                     /estimated_length=15
     gap             1056..1068
                     /estimated_length=13
     gap             1070..1080
                     /estimated_length=11
ORIGIN
        1 tnnnnagntg acgannngtc ctgtaagtta cgagcgtaga agcgatcacg gataccgtct
       61 accgcgtgag aatagtccct gatgctgcct tcagttttag ggcggggcag tacctaatgg
      121 ttgtgatgga tgaacgcgac aaacgaccgt ttagcatggc gagtaccccg gacgagaagg
      181 gctttattga actgcatatt ggcgcatcag aaatcaattt atatgcaaaa gctgtgatgg
      241 accgcattct gaaagatcac cagattgtcg ttgatatccc gcatggcgaa gcatggctgc
      301 gggatgatga agagcgtcca atgattctta tcgctggtgg cactggtttc agctacgccc
      361 ggtcgatatt gctcacagcg ctggcccgta acccgaaccg tgatattacc atctattggg
      421 ggggtcgtga agaacaacac ttgtatgatt tgtgcgagct ggaggccctg tcgctgaagc
      481 atcccggcct gcaggtagtg ccggtagtgg aacaaccaga ggctgggtgg cgtggacgca
      541 ctggcacagt tcttaccgca gtgctccagg accatggaac gttagccgaa cacgacattt
      601 atattgccgg tcgctttgag atggcaaaaa tcgcgcgcga tttattttgc tctgaacgta
      661 atgcgcgcga agaccgtctg ttcggtgatg cgttcgcgtt tatcacgcgt ggaggtggcc
      721 atcaccatca tcaccatcac cacggataac tcgagcaaag cccgccgaaa ggcgggcttt
      781 tctgtacaac aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt
      841 tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gatnnnnntc cngnntggcg
      901 taatagcgaa ganncccgcn ccgatcgccc ntncccanca gtnnncannn gaatgnnann
      961 gnngccnccc ngtagcgnnc atnncncgnn ggngngnnnn ncncncagcg ngacgctann
     1021 ntngcnagnn ncnagcnnnn nnnnnnnnnn ntnctnnnnn nnnnnnnncn nnnnnnnnnn
     1081 tnncnnnnnn nntnnnnann ngnnnnnnnc
//
