LOCUS       sequence-365574-        1102 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Fre via a T7 promoter in TXTL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pTXTL-T7max-Fre
FEATURES             Location/Qualifiers
     source          1..1102
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             765..787
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(795..814)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(796..814)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gap             1033..1048
                     /estimated_length=16
     gap             1065..1077
                     /estimated_length=13
     gap             1084..1098
                     /estimated_length=15
ORIGIN
        1 cnnnntannn tggngtggng angatggtga tggccacctc cacgcgtgat aaacgcgaac
       61 gcatcaccga acagacggtc ttcgcgcgca ttacgttcag agcaaaataa atcgcgcgcg
      121 atttttgcca tctcaaagcg accggcaata taaatgtcgt gttcggctaa cgttccatgg
      181 tcctggagca ctgcggtaag aactgtgcca gtgcgtccac gccacccagc ctctggttgt
      241 tccactaccg gcactacctg caggccggga tgcttcagcg acagggcctc cagctcgcac
      301 aaatcataca agtgttgttc ttcacgaccc ccccaataga tggtaatatc acggttcggg
      361 ttacgggcca gcgctgtgag caatatcgac cgggcgtagc tgaaaccagt gccaccagcg
      421 ataagaatca ttggacgctc ttcatcatcc cgcagccatg cttcgccatg cgggatatca
      481 acgacaatct ggtgatcttt cagaatgcgg tccatcacag cttttgcata taaattgatt
      541 tctgatgcgc caatatgcag ttcaataaag cccttctcgt ccggggtact cgccatgcta
      601 aacggtcgtt tgtcgcgttc atccatcaca accattaggt actgccccgc cctaaaactg
      661 aaggcagcat cagggactat tctcacgcgg tagacggtat ccgtgatcgc ttctacgctc
      721 gtaactttac aggacagggt cgtcatacta gtcataccgg tatatctcct tcttaaagtt
      781 aaacaaaatt atttccctat agtgagtcgt attagaatta gatctgtcga gccatgtgag
      841 caaaaggcca gcaaaagggc caggnaaccg taaaaaggcc gcgttgctgg ngtttttccc
      901 ataggctccc gcccccctga cgancatcac aaaaatcgac gctcantcag anngcgaaac
      961 ccgacaggac tnatnnnnta ccnnngtttc ccccnnnnnn ctncccnncn ngcgnctnnn
     1021 nctnnnnnna ncnnnnnnnn nnnnnnnncn nncnnnttnt ccntnnnnnn nnnnnnntnn
     1081 nngnnnnnnn nnnnnnnnct nc
//
