LOCUS       sequence-365575-        1122 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Firefly luciferase via a T7 promoter in TXTL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pTXTL-T7max-FLuc
FEATURES             Location/Qualifiers
     source          1..1122
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(69..87)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     gap             1087..1099
                     /estimated_length=13
ORIGIN
        1 agtnnggagn ngccaaaaac ntaaagaaag gcccggcgcc attctatccg ctggaagatg
       61 gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa
      121 ttgcttttac agatgcacat atcgaggtgg acatcactta cgctgagtac ttcgaaatgt
      181 ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg
      241 tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag
      301 ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgggca
      361 tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc
      421 aaaaaaagct cccaatcatc caaaaaatta ttatcatgga ttctaaaacg gattaccagg
      481 gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg
      541 attttgtgcc agagtccttc gatagggaca agacaattgc actgatcatg aactcctctg
      601 gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc gtgagattct
      661 cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg
      721 ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat
      781 ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tctgaggagc cttcaggatt
      841 acaagattca aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc aaaagcactc
      901 tgattgacaa tacgatttat ctaatttaca cgaaattgct tnctggnggn gctcccctct
      961 ctannnantc gggggaagcg gtngnccaan naggntccat nntgcnngta tcaggcnang
     1021 gnnnntnggg ctcactgana ctacatnnag nntnntnntg nnnnnccnnn nnnggnnnnt
     1081 nnatnannnn nnnnnnnnng nnnannnnnn cnttttttnn aa
//
