LOCUS       sequence-365576-        1123 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Firefly luciferase via a T7 promoter in TXTL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pTXTL-T7max-FLuc
FEATURES             Location/Qualifiers
     source          1..1123
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             1083..1092
                     /estimated_length=10
ORIGIN
        1 ctcnnnttcn tggtgagntg atgatggtga tggccacctc cacgcgtcac ggcgatcttt
       61 ccgcccttct tggcctttat gaggatctct ctgatttttc ttgcgtcgag ttttccggta
      121 agacctttcg gtacttcgtc cacaaacaca actcctccgc gcaacttttt cgcggttgtt
      181 acttgactgg cgacgtaatc cacgatctct ttttccgtca tcgtctttcc gtgctccaaa
      241 acaacaacgg cggcgggaag ttcaccggcg tcatcgtcgg gaagacctgc gacacctgcg
      301 tcgaagatgt tggggtgttg gagcaagatg gattccaatt cagcgggagc cacctgatag
      361 cctttgtact taatcagaga cttcaggcgg tcaacgatga agaagtgttc gtcttcgtcc
      421 cagtaagcta tgtctccaga atgtagccat ccatccttgt caatcaaggc gttggtcgct
      481 tccggattgt ttacataacc ggacataatc ataggacctc tcacacacag ttcgcctctt
      541 tgattaacgc ccagcgtttt cccggtatcc agatccacaa ccttcgcttc aaaaaatgga
      601 acaactttac cgaccgcgcc cggtttatca tccccctcgg gtgtaatcag aatagctgat
      661 gtagtctcag tgagcccata tccttgcctg atacctggca gatggaacct cttggcaacc
      721 gcttccccga cttccttaga gaggggagcg ccaccagaag caatttcgtg taaattagat
      781 aaatcgtatt tgtcaatcag agtgcttttg gcgaagaagg agaatagggg ttggcaccag
      841 cagcgcactt tgaatcttgt antcctgaaa ggctcctcag aaacagctct tcttcaaatc
      901 tatacattna agacgactcg gaaatccaca tntcaaatat nccganngta gtaaacattc
      961 ccaaaaccgg tgntggnaat gnnnnnacac ttaaaaatcg cagtatcngg aannattttg
     1021 atnnncnnaa aannngntcn ntgggcatgc nannatntca cncaggnnnn nnntatnnnn
     1081 nannnnnnnn nntnnnnnna nnnnnnanat nccnnaanng naa
//
