LOCUS       sequence-365577-        1124 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Renilla luciferase via a T7 promoter in TXTL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pTXTL-T7max-RLuc
FEATURES             Location/Qualifiers
     source          1..1124
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             2..11
                     /estimated_length=10
     gap             1085..1094
                     /estimated_length=10
     gap             1106..1123
                     /estimated_length=18
ORIGIN
        1 cnnnnnnnnn ntagtnngac ttcnaagttt atgatccaga acaaaggaaa cggatgataa
       61 ctggtccgca gtggtgggcc agatgtaaac aaatgaatgt tcttgattca tttattaatt
      121 attatgattc agaaaaacat gcagaaaatg ctgttatttt tttacatggt aacgcggcct
      181 cttcttattt atggcgacat gttgtgccac atattgagcc agtagcgcgg tgtattatac
      241 cagaccttat tggtatgggc aaatcaggca aatctggtaa tggttcttat aggttacttg
      301 atcattacaa atatcttact gcatggtttg aacttcttaa tttaccaaag aagatcattt
      361 ttgtcggcca tgattggggt gcttgtttgg catttcatta tagctatgag catcaagata
      421 agatcaaagc aatagttcac gctgaaagtg tagtagatgt gattgaatca tgggatgaat
      481 ggcctgatat tgaagaagat attgcgttga tcaaatctga agaaggagaa aaaatggttt
      541 tggagaataa cttcttcgtg gaaaccatgt tgccatcaaa aatcatgaga aagttagaac
      601 cagaagaatt tgcagcatat cttgaaccat tcaaagagaa aggtgaagtt cgtcgtccaa
      661 cattatcatg gcctcgtgaa atcccgttag taaaaggtgg taaacctgac gttgtacaaa
      721 ttgttaggaa ttataatgct tatctacgtg caagtgatga tttaccaaaa atgtttattg
      781 aatcggaccc aggattcttt tccaatgcta ttgttgaagg tgccaagaag tttcctaata
      841 ctgaatttgt caaagtaaaa ggtcttcatt ttttcgcaag aagatgcacc tgatgaaatg
      901 ggaaaatata tcaaatcgtt cgttgagcga gttctcaaaa atgnacnaac gcgtgnnngt
      961 gnnnatcacc atcatcaccc atcannnnga nnntcnagca ancccnnnnn aagcgggntt
     1021 tnctgtannn nacanngcat nnattnatgt tnnnggnncn nnnnnnatnn nnnnnntttn
     1081 aagcnnnnnn nnnnctannn nnnngnnnnn nnnnnnnnnn nnnc
//
