LOCUS       sequence-365578-        1135 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Renilla luciferase via a T7 promoter in TXTL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pTXTL-T7max-RLuc
FEATURES             Location/Qualifiers
     source          1..1135
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             1097..1107
                     /estimated_length=11
ORIGIN
        1 tgctcnnntt nngtggtgag ntgatgatgg tgatggccac ctccacgcgt ttgttcattt
       61 ttgagaactc gctcaacgaa cgatttgata tattttccca tttcatcagg tgcatcttct
      121 tgcgaaaaat gaagaccttt tactttgaca aattcagtat taggaaactt cttggcacct
      181 tcaacaatag cattggaaaa gaatcctggg tccgattcaa taaacatttt tggtaaatca
      241 tcacttgcac gtagataagc attataattc ctaacaattt gtacaacgtc aggtttacca
      301 ccttttacta acgggatttc acgaggccat gataatgttg gacgacgaac ttcacctttc
      361 tctttgaatg gttcaagata tgctgcaaat tcttctggtt ctaactttct catgattttt
      421 gatggcaaca tggtttccac gaagaagtta ttctccaaaa ccattttttc tccttcttca
      481 gatttgatca acgcaatatc ttcttcaata tcaggccatt catcccatga ttcaatcaca
      541 tctactacac tttcagcgtg aactattgct ttgatcttat cttgatgctc atagctataa
      601 tgaaatgcca aacaagcacc ccaatcatgg ccgacaaaaa tgatcttctt tggtaaatta
      661 agaagttcaa accatgcagt aagatatttg taatgatcaa gtaacctata agaaccatta
      721 ccagatttgc ctgatttgcc cataccaata aggtctggta taatacaccg cgctactggc
      781 tcaatatgtg gcacaacatg tcgccataaa taagaagagg ccgcgttacc atgtaaaaaa
      841 aataacagca ttttctgcat gttttttctg aatcataata attaataaat gaatcaagaa
      901 cattcatttg tttacatctg gcccaccact gcggaccagt tatcatccgt ttcctttgtt
      961 ctggatcata aactttcgaa ngtcatacta gtcataccgg tatatctcct tncnnnaagt
     1021 tnaaacnann tnnttnccta tanngagtcg taattanaan tnagatcnnn cnnannnntg
     1081 ngnnncnnaa nnnnncnnnn nnnnnnncng gnnnnnnaaa aaagnnngnn nnnng
//
