LOCUS       sequence-365580-        1140 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of NanoLuc via a T7 promoter in TXTL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pTXTL-T7max-NanoLuc
FEATURES             Location/Qualifiers
     source          1..1140
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(53..565)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     RBS             584..606
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(614..633)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(615..633)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(792..811)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     gap             1079..1088
                     /estimated_length=10
     gap             1110..1120
                     /estimated_length=11
ORIGIN
        1 ttngctcnnn tanngtggtg agntgatgat ggtgatggcc acctccacgc gtcgccagaa
       61 tgcgttcgca cagccgccag ccggtcactc cgttgatggt tactcggaac agcagggagc
      121 cgtcggggtt gatcaggcgc tcgtcgataa ttttgttgcc gttccacagg gtccctgtta
      181 cagtgatctt tttgccgtcg aacacggcga tgccttcata cggccgtccg aaatagtcga
      241 tcatgttcgg cgtaaccccg tcgattacca gtgtgccata gtgcaggatc accttaaagt
      301 gatgatcatc cacagggtac accaccttaa aaattttttc gatctggccc atttggtcgc
      361 cgctcagacc ttcatacggg atgatgacat ggatgtcgat cttcagccca ttttcaccgc
      421 tcaggacaat cctttggatc ggagttacgg acaccccgag attctgaaac aaactggaca
      481 cacctccctg ttcaaggact tggtccaggt tgtagccggc tgtctgtcgc cagtccccaa
      541 cgaaatcttc gagtgtgaag accatactag tcataccggt atatctcctt cttaaagtta
      601 aacaaaatta tttccctata gtgagtcgta ttagaattag atctgtcgag ccatgtgagc
      661 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
      721 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
      781 gacaggacta taaagatacc aggcgtttcc cccctggaag ctccctcgtg cgctctcctg
      841 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      901 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
      961 ggctgtgtgc acgaaacccc cccgtttcag cccgaccgct gcgccnttat ncnggtaant
     1021 atcgtcttng antnccancc cgggtaagan ncnacttntc nnnntnnnnn agccnactnn
     1081 nnnnnnnntn anccanannn nnnnnntgtn nnnnnnnnnn ttacnnnnnn ntctttnnna
//
