LOCUS       sequence-365581-        1070 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of eGFP-Luz via a T7 promoter in TXTL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pTXTL-T7max-HisGFPLuz
FEATURES             Location/Qualifiers
     source          1..1070
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(73..94)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(334..353)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     gap             1060..1069
                     /estimated_length=10
ORIGIN
        1 gnnnctnncc atcntcacca tcaccacggn ggtggcacta gtatggagct tttcactggc
       61 gttgttccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      121 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      181 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
      241 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      301 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      361 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      421 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
      481 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
      541 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      601 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
      661 cccaacgaga agcgcgatca catggtcctg ctgggagttc gtgaccgccg ccgggatcgg
      721 aggtggtgga tcgggcggga gggggatcaa tgcgtatcaa catctccctg tcgtcgttat
      781 tcgaacggtt aagtaaactg agctctcgtt caattgcgat tacctgntgg gagtttgtcc
      841 tcgccctctg ctattgcttt tcccatcata cgtcgggntt tatcagacgt tcctgggang
      901 ttnggtncnt anctatgccc cgnaaaaacn nnncnggcta cattatcgnt tgcgtacttt
      961 agcttgnnnn nnnnncagaa annnctnggn gatctacgan cngntccaga nnnnnnnntg
     1021 ntnnnngntc nnnnttnnnn nnnnnaccan annnnntntn nnnnnnnnnt
//
