LOCUS       sequence-365582-        1078 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of eGFP-Luz via a T7 promoter in TXTL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pTXTL-T7max-HisGFPLuz
FEATURES             Location/Qualifiers
     source          1..1078
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     18..39
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     gap             1023..1032
                     /estimated_length=10
     gap             1062..1071
                     /estimated_length=10
ORIGIN
        1 cnnnnnnanc gcgancacat ggtcctgctg gagttcgtga ccgccgccgg gatcggaggt
       61 ggtggatcgg gcggaggggg atcaatgcgt atcaacatct ccctgtcgtc gttattcgaa
      121 cggttaagta aactgagctc tcgttcaatt gcgattacct gtggagttgt cctcgcctct
      181 gctattgctt ttcccatcat acgtcgggat tatcagacgt tcctggaggt tggtcctagc
      241 tatgccccgc aaaactttcg cggctacatt atcgtttgcg tacttagctt gttccgccaa
      301 gagcagaaag gcctggcgat ctacgaccgg ctcccagaga agcgccgctg gctggcggat
      361 ctgccatttc gtgaaggtac cagaccgagt attacctcac acattatcca gcgccagcgc
      421 actcaacttg tggatcagga atttgccaca cgtgaactga ttgacaaggt tattccgagg
      481 gtgcaagcac gacataccga taaaacattt ttgagcacga gcaagtttga atttcatgcg
      541 aaagccattt tcctgttgcc atcgattcca atcaacgacc cgctgaacat acctagtcat
      601 gatacggtgc gtcgaacgaa acgcgaaatt gcccacatgc atgactatca cgattgcacc
      661 cttcacctag ctctggcggc acaggatgga aaaagaagtc ctgaaaaaag gctggggcca
      721 gcgtcatccg ctggcggggc cgggtgtgcc tgggccgccg actgaatgga ctttcttata
      781 cgcaccccgt aatgaggaag aagcgcgcgt ggtanaaatg attgtgggaa gcatccatcg
      841 gctatatgac caatgatccg gcaggtaaaa tcgtcgagaa tgncantann gcgttnaact
      901 cgagcnnngc ccnnnatcan catcatcanc atcaccacgn gnggcactan tgnnnnntac
      961 gacncgnnnt cnagnnaagc ccnnnnannn nnnntttntn gtacanacat tgcattcntn
     1021 ntnnnnnnnn nncnnnnnna nnnngnannn nnnnncagtn annnnnnnnn ngnnnnna
//
