LOCUS       sequence-365583-        1140 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Luz-eGFP via a T7 promoter in TXTL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pTXTL-T7max-LuzGFPHis
FEATURES             Location/Qualifiers
     source          1..1140
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     227..246
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        227..245
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             254..276
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     gap             1100..1109
                     /estimated_length=10
     gap             1122..1131
                     /estimated_length=10
ORIGIN
        1 cnnnagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
       61 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
      121 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
      181 ggccttttgc tggccttttg ctcacatggc tcgacagatc taattctaat acgactcact
      241 atagggaaat aattttgttt aactttaaga aggagatata ccggtatgac tagtatgcgt
      301 atcaacatct ccctgtcgtc gttattcgaa cggttaagta aactgagctc tcgttcaatt
      361 gcgattacct gtggagttgt cctcgcctct gctattgctt ttcccatcat acgtcgggat
      421 tatcagacgt tcctggaggt tggtcctagc tatgccccgc aaaactttcg cggctacatt
      481 atcgtttgcg tacttagctt gttccgccaa gagcagaaag gcctggcgat ctacgaccgg
      541 ctcccagaga agcgccgctg gctggcggat ctgccatttc gtgaaggtac cagaccgagt
      601 attacctcac acattatcca gcgccagcgc actcaacttg tggatcagga atttgccaca
      661 cgtgaactga ttgacaaggt tattccgagg gtgcaagcac gacataccga taaaacattt
      721 ttgagcacga gcaagtttga atttcatgcg aaagccattt tcctgttgcc atcgattcca
      781 atcaacgacc cgctgaacat acctagtcat gatacggtgc gtcgaacgaa acgcgaaatt
      841 gcccacatgc atgactatca cgattgcacc cttcacctag ctctggcggc acagggatgg
      901 gaaaagaagt cctgaaaaaa annctggggg ccagcgtcat ccgctggncg ggnnngggtg
      961 tgccctgnnc gccgactgaa tnnnctttct tatacnccac ccccgtaatg annnnnncgc
     1021 gncgtggnna naaanngatg nnnnncatcc catcnggnta tntngannnn gantccgggc
     1081 nnnnaaannc gnncganaan nnnnnnnnng nngnntcnnn gnnnnnnnnn ncantnggna
//
