LOCUS       sequence-365584-        1092 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Luz-eGFP via a T7 promoter in TXTL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pTXTL-T7max-LuzGFPHis
FEATURES             Location/Qualifiers
     source          1..1092
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(143..164)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(404..423)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     751..772
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             803..826
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     gap             1065..1074
                     /estimated_length=10
ORIGIN
        1 cgcgcgtggt agantgattg tggaagcatc catcggctat atgaccaatg atccggcagg
       61 taaaatcgtc gagaatgcca aaggaggtgg tggatcgggc ggagggggat caatggagct
      121 tttcactggc gttgttccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
      181 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
      241 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
      301 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
      361 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
      421 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
      481 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
      541 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
      601 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
      661 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
      721 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
      781 cgggatcacg cgtggaggtg gccatcacca tcatcaccat caccacggat aactcgagca
      841 aagcccgccg aaagggcggg cttttctgta caacaacaat tgcattcatt ttatgtttcn
      901 gnttcnnggg gagatgtggg aggttttttn aagcaagtaa aaacctctac aatgtggnaa
      961 aaatcgataa gnatccgggn tggnntaann ancgaagann ncgcaccgat cgnccnncca
     1021 nannncgcag ccntgannnn ganngncgcn cccnnnancg nngcnnnnnn nnnngnnnnn
     1081 nngtacnnnc ag
//
