LOCUS       sequence-365585-        1129 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of HiBiT-GFP via T7 promoter in TXTL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pTXTL-T7max-HiBiTGFPHis
FEATURES             Location/Qualifiers
     source          1..1129
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     227..246
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        227..245
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             254..276
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(391..412)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(652..671)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     gap             1107..1117
                     /estimated_length=11
ORIGIN
        1 tcggtagcgg cagggtcgga cagganagcg cacgagggag cttccagggg gaaacgcctg
       61 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
      121 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
      181 ggccttttgc tggccttttg ctcacatggc tcgacagatc taattctaat acgactcact
      241 atagggaaat aattttgttt aactttaaga aggagatata ccggtatgac tagtatggtg
      301 agcggctggc ggctgttcaa gaagattagc ggaggtggtg gatcgggcgg agggggatca
      361 atggagcttt tcactggcgt tgttcccatc ctggtcgagc tggacggcga cgtaaacggc
      421 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      481 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
      541 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
      601 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
      661 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      721 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
      781 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
      841 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
      901 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
      961 tccgccctga gcaaagaccc caacganaag cgcnatcaca tggncctgct ggnnttcgtg
     1021 nncgccnccn gggnncacnn nnnnngtgnn ccatncacca tcatcnncnn tcnnnnncgg
     1081 nnnactcnnn gncannnnnn nncnnannnn nnnnnnncnn nnnncaanc
//
