LOCUS       sequence-365589-        1173 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of LgBiT via sigma 70 promoter in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pLumi-LgBiT
FEATURES             Location/Qualifiers
     source          1..1173
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     668..688
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(900..919)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     gap             1137..1149
                     /estimated_length=13
ORIGIN
        1 atnntnnnnn ntagnnnnna ncaggggatc ctctagagtc gacctgcagg tcgacggatc
       61 cggggaattc aggcttggag gatacgtatg gtcttcacac tcgaagattt cgttggggac
      121 tgggaacaga cagccgcgta caacctggac caagtccttg aacagggagg tgtgtccagt
      181 ttgctgcaga atctcgcggt gtccgtaact ccgatccaaa ggattgtccg cagcggtgaa
      241 aatgcgctga agatcgacat ccatgtcatc atcccgtatg aaggtctgag cgcggaccaa
      301 atggcacaga tcgaagaagt gtttaaggtg gtgtaccctg tggatgatca tcactttaag
      361 gtgatcctgc cgtatggcac actggtaatc gacggggtta cgccgaacat gctgaactat
      421 ttcggacggc cgtatgaagg catcgccgtg ttcgacggca aaaagatcac tgtaacaggg
      481 accctgtgga acggcaacaa aattatcgac gagcgcctga tcacccccga cggctccatg
      541 ctgttccgag taaccatcaa cagctgacct gcaggcatgc aagcttgcgg ccgcgggccc
      601 catggatcga tagctggtcg acacaatctg ccctttcgaa agatcccaac gaaaagcgtg
      661 accacatggt ccttcttgag tttgtaactg ctgctgggat tacacatggc atggatgagc
      721 tctacaaata atgagctagc ccgcctaatg agcgggcttt tttttctcgg cctaggagat
      781 acttaacagg gaagtgagag ggccgcggca aagccgtttt tccataggct ccgccccccc
      841 tgacaagcat cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga caggactata
      901 aagataccag gcgtttcccc cctggnggct ccccccgtgc gctctcctgn ttcctgcctt
      961 tcgggtttac cggtgtcatt ccgctgttat ggcccgcgtt tgtctcattn ccacgccctg
     1021 acactcantt ccggggtang cagttcgctc ccaagntgga ctgtatgcac nnnccccccn
     1081 cnntcannnn nancgntngc nnnttatccg gnnactatcg tcttngannn nnnnncnnnn
     1141 nnnnnnnnnc nnnaaannnn nncccnncnt gng
//
