LOCUS       sequence-365590-        1156 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of LgBiT via sigma 70 promoter in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pLumi-LgBiT
FEATURES             Location/Qualifiers
     source          1..1156
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(624..671)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 gtgtcgacca gctatcgatc catggggccc gcggccgcaa gcttgcatgc ctgcaggtca
       61 gctgttgatg gttactcgga acagcatgga gccgtcgggg gtgatcaggc gctcgtcgat
      121 aattttgttg ccgttccaca gggtccctgt tacagtgatc tttttgccgt cgaacacggc
      181 gatgccttca tacggccgtc cgaaatagtt cagcatgttc ggcgtaaccc cgtcgattac
      241 cagtgtgcca tacggcagga tcaccttaaa gtgatgatca tccacagggt acaccacctt
      301 aaacacttct tcgatctgtg ccatttggtc cgcgctcaga ccttcatacg ggatgatgac
      361 atggatgtcg atcttcagcg cattttcacc gctgcggaca atcctttgga tcggagttac
      421 ggacaccgcg agattctgca gcaaactgga cacacctccc tgttcaagga cttggtccag
      481 gttgtacgcg gctgtctgtt cccagtcccc aacgaaatct tcgagtgtga agaccatacg
      541 tatcctccaa gcctgaattc cccggatccg tcgacctgca ggtcgactct agaggatccc
      601 cgggtaccac tagtgggccc tgagtcgtat tatactatgc cgatatacta tgccgatgat
      661 taattgtcaa cacgtccatg ggtttaaacg cggccgcgga tcccggtacc aagcttcata
      721 tgcccggggc atgccctgca ggaattccac atgagagttt cagaaaatga cagagcgtga
      781 gcaagtgcct ttattgaaat tgcctgaata acagagacag tgcaaacgat tatttcataa
      841 taacgattat gtaatgactg gtaagaaaat aacttgnttg agttcccttt atccagcctg
      901 atagtggata aaggggaact caataataat tgaattaatt tattagcttc atcgcatttt
      961 ttttttggtc tcttcatcac tttttttttg cctcntnnnn gtgaannngn nttgagngan
     1021 ctattgaaan aanttaaaaa tcggnnnnat ctcttttnnn naaatnnnnn tcnnnnnnta
     1081 ttnncnnata tcnnnaaaan ngnnnnntnn tntcnncann agcnnnnnca ttaaaanngn
     1141 nnnagggggg nnnncc
//
