LOCUS       sequence-36560-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  TITLE     George Cross lab plasmid
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLEW100V5-BSD
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    225..243
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    246..264
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     541..560
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        541..559
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cctttcttta tgtttttggc gtcttccatt ttaccaacag taccggaatg ctaatgtcac
       61 tgcgagcgac attcaagaat gtaacacaaa ggaattccaa gcttgtgaat tttacttttt
      121 ggtgtaattg aagtcttcaa gttgaaaaaa atggggaaaa ggaaaaaaaa ctcgaaggcg
      181 cgtgcagccc gggacgagaa catgttaaac tcagtactca gggatctcta tcactgatag
      241 ggagatctct atcactgata gggaaccgta cgccgtaagc gctactttta ctgcttaatc
      301 tttcctatat aagatatatt tctattacta tatatacttt tttaacttta tcattattat
      361 cctattgtgt gttgtattga ctttacaata aaacatatta tatctaacat gtcaaaagca
      421 tcggctcttc aatacagtta cccgtccctt atcaacacag tacaatatac tctgtcatga
      481 caataagtat atataagagc cagaatgcac ccgcgctggg tggaaagcta ggtacctgat
      541 taatacgact cactataggg agatctcccg taccgggggc acagcaaggt cttctgaaat
      601 tcatgttttt ttttttttac tctgcattgc agtctccgct cttatttagt tttgctttac
      661 gtaaggtctc gttgctgcca taaaataagc tatcaccatg gccaagcctt tgtctcaaga
      721 agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga
      781 ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt
      841 atatcatttt actgggggac cttg
//
