LOCUS       sequence-365603-        6817 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Sakai H, Yasugi T, Benson JD, Dowhanick JJ, Howley PM
  TITLE     Targeted mutagenesis of the human papillomavirus type 16 E2 
            transactivation domain reveals separable transcriptional activation 
            and DNA replication functions.
  JOURNAL   J Virol. 1996 Mar . 70(3):1602-11.
  PUBMED    8627680
REFERENCE   2  (bases 1 to 6817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            1996 Mar . 70(3):1602-11."
COMMENT     Sequence Label: p3692 HPV-16 E1
FEATURES             Location/Qualifiers
     source          1..6817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      703..712
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2668..3290
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2867..2886)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(3314..3334)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3319..3648
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3499..3634
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          3687..3783
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     complement(3859..3878)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3860..3878)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3892..3908)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3892..3908)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3905..3927)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3916..3932
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3940..3946,3947..3964,3965..3970))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3985..4006
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4123..4140)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4294..4882)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4374..4393)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5053..5844,5845..5913))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5676..5695
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5914..6018)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6045..6500
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6132..6151)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6342..6363
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6626..6648
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6640..6657
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6641..6657
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agaattgttt ttatttttaa ttttctttca aatacttcca tcgaattcag atctggtacc
      661 acgcgtatcg ataagcttgc atgcctgcag gtcgactcta gagccaccat ggctgatcct
      721 gcaggtacca atggggaaga gggtacggga tgtaatggat ggttttatgt agaggctgta
      781 gtggaaaaaa aaacagggga tgctatatca gatgacgaga acgaaaatga cagtgataca
      841 ggtgaagatt tggtagattt tatagtaaat gataatgatt atttaacaca ggcagaaaca
      901 gagacagcac atgcgttgtt tactgcacag gaagcaaaac aacatagaga tgcagtacag
      961 gttctaaaac gaaagtattt gggtagtcca cttagtgata ttagtggatg tgtagacaat
     1021 aatattagtc ctagattgaa agctatatgt atagaaaaac aaagtagagc tgcaaaaagg
     1081 agattatttg aaagcgaaga cagcgggtat ggcaatactg aagtggaaac tcagcagatg
     1141 ttacaggtag aagggcgcca tgagactgaa acaccatgta gtcagtatag tggtggaagt
     1201 gggggtggtt gcagtcagta cagtagtgga agtgggggag agggtgttag tgaaagacac
     1261 actatatgcc aaacaccact tacaaatatt ttaaatgtac taaaaactag taatgcaaag
     1321 gcagcaatgt tagcaaaatt taaagagtta tacggggtga gtttttcaga attagtaaga
     1381 ccatttaaaa gtaataaatc aacgtgttgc gattggtgta ttgctgcatt tggacttaca
     1441 cccagtatag ctgacagtat aaaaacacta ttacaacaat attgtttata tttacacatt
     1501 caaagtttag catgttcatg gggaatggtt gtgttactat tagtaagata taaatgtgga
     1561 aaaaatagag aaacaattga aaaattgctg tctaaactat tatgtgtgtc tccaatgtgt
     1621 atgatgatag agcctccaaa attgcgtagt acagcagcag cattatattg gtataaaaca
     1681 ggtatgtcaa atattagtga agtgtatgga gacacgccag aatggataca aagacaaaca
     1741 gtattacaac atagttttaa tgattgtaca tttgaattat cacagatggt acaatgggcc
     1801 tacgataatg acatagtaga cgatagtgaa attgcatata aatatgcaca attggcagac
     1861 actaatagta atgcaagtgc ctttctaaaa agtaattcac aggcaaaaat tgtaaaggat
     1921 tgtgcaacaa tgtgtagaca ttataaacga gcagaaaaaa aacaaatgag tatgagtcaa
     1981 tggataaaat atagatgtga tagggtagat gatggaggtg attggaagca aattgttatg
     2041 tttttaaggt atcaaggtgt agagtttatg tcatttttaa ctgcattaaa aagatttttg
     2101 caaggcatac ctaaaaaaaa ttgcatatta ctatatggtg cagctaacac aggtaaatca
     2161 ttatttggta tgagtttaat gaaatttctg caagggtctg taatatgttt tgtaaattct
     2221 aaaagccatt tttggttaca accattagca gatgccaaaa taggtatgtt agatgatgct
     2281 acagtgccct gttggaacta catagatgac aatttaagaa atgcattgga tggaaattta
     2341 gtttctatgg atgtaaagca tagaccattg gtacaactaa aatgccctcc attattaatt
     2401 acatctaaca ttaatgctgg tacagattct aggtggcctt atttacataa tagattggtg
     2461 gtgtttacat ttcctaatga gtttccattt gacgaaaacg gaaatccagt gtatgagctt
     2521 aatgataaga actggaaatc ctttttctca aggacgtggt ccagattaag tttgcacgag
     2581 gacgaggaca aggaaaacga tggagactct ttgccaacgt ttaaatgtgt gtcaggacaa
     2641 aatactaaca cattatgaaa atgacccggg tggcatccct gtgacccctc cccagtgcct
     2701 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt
     2761 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta
     2821 tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca
     2881 agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg
     2941 attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc
     3001 taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac
     3061 tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga
     3121 accactgctc ccttccctgt ccttctgatt ttaaaataac tataccagca ggaggacgtc
     3181 cagacacagc ataggctacc tggccatgcc caaccggtgg gacatttgag ttgcttgctt
     3241 ggcactgtcc tctcatgcgt tgggtccact cagtagatgc ctgttgaatt gggtacgcgg
     3301 ccagcttggc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     3361 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     3421 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     3481 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     3541 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     3601 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcctcgag
     3661 gaactgaaaa accagaaagt taactggtaa gtttagtctt tttgtctttt atttcaggtc
     3721 ccggatccgg tggtggtgca aatcaaagaa ctgctcctca gtggatgttg cctttacttc
     3781 taggcctgta cggaagtgtt acttctgctc taaaagctgc tgcaggtcga ctctagggcc
     3841 gcgtaccgag ctcgaattcc ctatagtgag tcgtattaaa ttcgtaatca tgtcatagct
     3901 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     3961 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     4021 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     4081 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     4141 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     4201 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     4261 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     4321 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     4381 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     4441 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     4501 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     4561 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     4621 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     4681 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     4741 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     4801 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     4861 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     4921 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     4981 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     5041 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     5101 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     5161 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     5221 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     5281 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     5341 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     5401 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     5461 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     5521 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     5581 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     5641 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     5701 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     5761 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     5821 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     5881 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     5941 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     6001 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg
     6061 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     6121 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     6181 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     6241 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     6301 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     6361 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     6421 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     6481 aatattaacg cttacaattt ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     6541 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     6601 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
     6661 aagctgatct atacattgaa tcaatattgg caattagcca tattagtcat tggttatata
     6721 gcataaatca atattggcta ttggccattg catacgttgt atctatatca taatatgtac
     6781 atttatattg gctcatgtcc aatatgaccg ccatgtt
//
