LOCUS       sequence-365606-       10265 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10265)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 10265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: TTC3_pCSdest
FEATURES             Location/Qualifiers
     source          1..10265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        39..1023
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        1058..1076
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1058..1075
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    1130..1154
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7272..7296)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    7340..7474
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7364..7383)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7418..7437
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7587..7607)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7587..7605)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7626..7642)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7626..7642)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7639..7661)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7650..7666
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7674..7680,7681..7698,7699..7704))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7719..7740
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7857..7874)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8028..8616)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8108..8127)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8787..9578,9579..9647))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9410..9429
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9648..9752)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9778..10233)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9915..9936)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10127..10146
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagt cgaccgccaa ttcaatatgg
       61 cgtatatgga ctcatgccaa ttcaatatgg tggatctgga cctgtgccaa ttcaatatgg
      121 cgtatatgga ctcgtgccaa ttcaatatgg tggatctgga ccccagccaa ttcaatatgg
      181 cggacttggc accatgccaa ttcaatatgg cggacctggc actgtgccaa ctggggaggg
      241 gtctacttgg cacggtgcca agtttgagga ggggtcttgg ccctgtgcca agtccgccat
      301 attgaattgg catggtgcca ataatggcgg ccatattggc tatatgccag gatcaatata
      361 taggcaatat ccaatatggc cctatgccaa tatggctatt ggccaggttc aatactatgt
      421 attggcccta tgccatatag tattccatat atgggttttc ctattgacgt agatagcccc
      481 tcccaatggg cggtcccata taccatatat ggggcttcct aataccgccc atagccactc
      541 ccccattgac gtcaatggtc tctatatatg gtctttccta ttgacgtcat atgggcggtc
      601 ctattgacgt atatggcgcc tcccccattg acgtcaatta cggtaaatgg cccgcctggc
      661 tcaatgccca ttgacgtcaa taggaccacc caccattgac gtcaatggga tggctcattg
      721 cccattcata tccgttctca cgccccctat tgacgtcaat gacggtaaat ggcccacttg
      781 gcagtacatc aatatctatt aatagtaact tggcaagtac attactattg gaagtacgcc
      841 agggtacatt ggcagtactc ccattgacgt caatggcggt aaatggcccg cgatggctgc
      901 caagtacatc cccattgacg tcaatgggga ggggcaatga cgcaaatggg cgttccattg
      961 acgtaaatgg gcggtaggcg tgcctaatgg gaggtctata taagcaatgc tcgtttaggg
     1021 aaccgccatt ctgcctgggg acgtcggagc aagcttgatt taggtgacac tatagaatac
     1081 aagctacttg ttctttttgc aggatcccat cgattcgaat tcaaggatca caagtttgta
     1141 caaaaaagca ggctccgcgg ccgccccctt caccatggac aattttgctg agggagattt
     1201 cactgtggcg gattatgcct tgttagaaga ttgccctcac gtggatgatt gtgtctttgc
     1261 tgctgaattt atgagcaatg attatgttcg tgtgactcag ctttactgtg atggggtggg
     1321 tgtgcaatat aaagattata tccaaagtga gaggaatttg gaatttgaca tctgcagtat
     1381 atggtgtagt aaaccaattt ctgtcctgca agattattgc gatgccatta aaataaacat
     1441 cttctggcca cttctgtttc aacatcaaaa cagttccgta atatcacgat tgcatccctg
     1501 tgtggacgcc aacaattcac gtgcttctga gataaatttg aagaaactac aacatcttga
     1561 gttgatggaa gatattgtgg atttggcaaa gaaagttgct aatgattcat tccttattgg
     1621 aggcttattg agaattggtt gtaaaataga aaataaaatc ttggcaatgg aagaagctct
     1681 gaattggata aaatatgcag gcgatgtaac aattctaact aaattaggat caattgacaa
     1741 ttgttggcct atgttaagta ttttctttac tgaatacaag taccacataa ctaaaattgt
     1801 aatggaagac tgcaatttgc ttgaagaact taaaactcaa agttgtatgg attgtataga
     1861 ggaaggagaa ctaatgaaaa tgaaaggaaa tgaagagttt tccaaagaaa gatttgatat
     1921 agctattatc tattacacca gagccattga atatagacct gaaaactacc ttctttatgg
     1981 taaccgagct ctttgttttc ttcgtactgg acagtttaga aatgcactcg gtgatggaaa
     2041 gagagccact attctgaaga acacttggcc aaagggtcat tatcgttatt gtgatgctct
     2101 ttctatgctg ggggaatatg actgggccct gcaagcaaac ataaaagctc aaaaactctg
     2161 taaaaatgac cctgagggaa tcaaggatct aattcagcag catgtaaagt tacaaaaaca
     2221 aatagaagac ctacaaggtc gaacagcaaa taaggatcca attaaagcct tttatgaaaa
     2281 cagggcctac acacctagga gtttatcagc acctatattt actacttcac ttaactttgt
     2341 ggagaaggaa agagatttca gaaaaattaa tcacgaaatg gccaacggtg gtaatcagaa
     2401 tctaaaggtg gcggatgagg cgttgaaggt agatgattgt gactgtcatc ctgaattttc
     2461 accaccatca agtcagcctc caaaacataa aggaaaacaa aaatctcgaa acaatgaatc
     2521 agaaaagttc agttctagtt caccattgac tttaccagca gatttgaaga acatcttgga
     2581 gaaacagttt tctaaatctt ccagagctgc acaccaggat tttgctaata taatgaaaat
     2641 gctgagaagc ttaattcaag atggctatat ggccttattg gagcagcgtt gccgcagcgc
     2701 tgcacaggcc tttacagagt tgctgaacgg tttagatcct caaaaaataa agcaattgaa
     2761 cctggccatg attaactatg ttttggtcgt ctatggactt gccatttctc tccttggaat
     2821 aggacagcct gaggaattat ctgaagccga aaaccagttt aagaggatta ttgaacacta
     2881 ccccagtgag ggccttgatt gcttggccta ctgtggaatt ggaaaagtat atttgaaaaa
     2941 aaacagattt ctagaagctc tcaatcactt tgagaaagca agaaccttga tttatcgtct
     3001 tcctggagtg ttaacttggc ccacgagtaa tgtgattatt gaagagtctc agccacaaaa
     3061 aataaagatg ctgttagaga aatttgttga agaatgcaag ttccctccag tgccagatgc
     3121 catttgttgc tatcagaagt gccatggata ttctaagatc cagatataca taactgatcc
     3181 agactttaag ggttttatac gcatcagctg ttgccagtac tgtaaaatag aatttcacat
     3241 gaattgctgg aagaagttaa aaactacaac ctttaatgat aaaattgaca aggattttct
     3301 acaaggaata tgtcttaccc ctgactgtga aggtgtcatt tctaagatta tcatcttcag
     3361 cagtggtggt gaagttaaat gtgaatttga acacaaggtc ataaaagaaa aggttcctcc
     3421 aagacctatt ctgaaacaga aatgttctag cctagagaaa ctaagactga aagaagacaa
     3481 aaaattgaag agaaagatcc aaaaaaaaga agcaaaaaag ttagcacaag aaagaatgga
     3541 ggaggactta agagaaagta atccacccaa aaatgaagag cagaaagaaa ctgtagacaa
     3601 tgttcagcgt tgtcagttcc ttgatgacag aattctacag tgtataaagc agtatgctga
     3661 caagattaaa tccggcatac agaatacagc catgcttctc aaagaattgc tttcttggaa
     3721 agttttgagc acagaagact atacaacctg tttttctagc agaaattttc taaatgaagc
     3781 agtggactat gttattcgcc acttgattca agaaaataac agagtaaaga caagaatatt
     3841 tctgcatgtt ttgagtgagc ttaaagaagt ggagcccaaa ttagccgcct ggatccaaaa
     3901 acttaatagc tttggcttag atgccacagg aactttcttt tctcgttatg gagcatctct
     3961 taaactgctt gattttagta tcatgacttt cctctggaat gagaaatatg gtcacaaact
     4021 agactctata gaaggaaagc aacttgatta tttctctgag ccagcatcat tgaaggaagc
     4081 ccgttgttta atatggctgc tagaagaaca cagagacaag ttcccagcat tgcatagtgc
     4141 tttagatgaa ttctttgata taatggacag ccgctgtact gtgttaagga aacaagatag
     4201 tggtgaagca ccgtttagtt caaccaaggt gaaaaacaaa agcaagaaaa agaagccaaa
     4261 ggattcaaag cctatgttag ttgggtctgg aacaacttca gtaacttcaa ataatgagat
     4321 catcacttca agtgaagacc atagcaatcg aaattcagat tctgcaggcc catttgcagt
     4381 gcctgaccat cttcggcaag atgtagaaga attcgaagct ctctatgacc aacacagtaa
     4441 cgaatatgtt gtccgcaata agaagctatg ggacatgaac ccaaaacaaa aatgttcaac
     4501 tctatatgat tacttctctc agtttttgga ggaacatggt cccttggaca tgagtaacaa
     4561 gatgttctct gcagaatatg agtttttccc agaagaaact cgacagatac tagaaaaagc
     4621 aggaggttta aaaccttttc tcttgggatg ccctcgtttt gttgtgattg acaactgtat
     4681 tgcactgaag aaggttgcat cacggctcaa gaaaaaaagg aagaagaaaa acattaaaac
     4741 aaaagtagaa gaaatttcaa aagcagggga gtatgtacga gttaaactac aactgaatcc
     4801 agctgctagg gaatttaaac cagatgtaaa gtctaaacca gtgtcagatt catcttcagc
     4861 accagctttt gaaaatgtga aacccaaacc tgtgtctgca aattctccca agccagcttg
     4921 tgaagatgtg aaggccaaac cagtatccga caattcttct agacaagttt ctgaggatgg
     4981 gcaacccaaa ggggtctctt ctaattctcc taaaccaggc tctgaggatg caaattacaa
     5041 gcgagtctcc tgtaattccc ccaaaccggt tcttgaggat gtgaaaccaa cttattgggc
     5101 tcaatcccat ttggtcacag gatactgtac gtatcttcct ttccagagat ttgatatcac
     5161 ccagacaccg ccagcataca taaacgtgtt accaggtttg ccccagtaca ccagcatata
     5221 tacacccttg gccagccttt ctcctgaata tcagctacca agatcagtac cagtggtgcc
     5281 gtcttttgta gccaatgaca gagcagataa aaatgctgct gcctattttg agggtcatca
     5341 tttgaatgct gagaatgttg ctggtcacca gattgcctct gaaacacaga tccttgaggg
     5401 ctctttggga atatctgtaa agtcacactg cagcacaggt gatgctcata cagtcctgag
     5461 tgagtctaac agaaatgatg agcactgtgg aaattctaac aacaaatgtg aagtaattcc
     5521 agaaagcacc agtgcagtaa caaacattcc acacgtgcag atggttgcca tacaggtatc
     5581 ttggaacata atacaccaag aagtcaatac tgagccatat aatccttttg aggaacgaca
     5641 aggggaaatt tcacggattg aaaaggagca ccaagtatta caagaccaac ttcaagaagt
     5701 gtatgaaaat tatgagcaga taaaacttaa gggcttagaa gagaccaggg acctggaaga
     5761 gaagttgaaa aggcacttag aagaaaacaa gatctcaaag acggaattag attggttcct
     5821 tcaagatttg gaaagagaaa ttaaaaaatg gcaacaggaa aaaaaagaaa tccaagaaag
     5881 actaaaatca ctgaagaaga aaattaaaaa ggtttcaaat gccagtgaaa tgtataccca
     5941 gaaaaatgat ggaaaggaaa aggaacatga attacatctg gatcagtccc ttgaaatcag
     6001 caacacactt acaaatgaga aaatgaaaat agaagagtat ataaagaaag ggaaagagga
     6061 ttatgaagag agtcatcaga gagctgtggc tgcagaggta tccgtacttg aaaactggaa
     6121 ggagagtgaa gtgtataagc tacagatcat ggagtcacaa gcagaagcct ttctgaagaa
     6181 gctggggctg attagccgtg atcctgcagc atatcctgac atggagtctg atatacgttc
     6241 atgggaattg tttctttcta atgttacaaa agaaattgag aaagcaaagt ctcagtttga
     6301 agaacaaatt aaggcaatta aaaatggttc tcggctcagt gaactttcta aagtgcagat
     6361 ttctgagctt tcatttcctg cctgtaacac ggttcatccc gagttactcc ctgagtcttc
     6421 aggcgacgat ggccaagggc ttgtgacttc tgcaagcgac gtgactggaa accacgcagc
     6481 acttcacagg gatcctagtg tgttctctgc tggtgattcc ccaggggagg ctccttctgc
     6541 gctgttgcca gggccacccc ctggtcagcc tgaagccact cagctgacag ggccaaaacg
     6601 ggctggccag gcagctctgt cagaacgaag ccctgtggct gatcggaagc agcctgttcc
     6661 tccaggacgt gctgcgcgtt caagccagtc tccaaaaaag ccgttcaata gtattattga
     6721 gcacctgtca gtggtattcc catgttacaa cagcactgag cttgctggtt ttattaaaaa
     6781 agtgcgaagc aaaaacaaga actcactctc aggattgagt attgatgaaa ttgtccaaag
     6841 agtgacagaa cacattctag atgaacagaa aaagaaaaag ccaaacccag gaaaggacaa
     6901 gaggacttat gagcccagct ctgccacccc cgtgaccagg tcctcccagg gctcaccctc
     6961 ggtggttgtt gcaccatcac ccaaaaccaa ggggcagaaa gcagaagatg tccctgtgag
     7021 gattgcactg ggtgcaagtt cctgtgaaat atgccacgag gtgttcaaat caaaaaacgt
     7081 gcgtgtgctc aaatgtgggc acaagtatca caaagggtgc tttaagcagt ggcttaaagg
     7141 gcagagcgct tgcccggcct gccagggtcg tgatctcctg acagaagagt caccttctgg
     7201 aagaggctgg cccagtcaga atcaggagct gccttcctgc tcttctaggt agtcaaaggg
     7261 tgggcgcgcc gacccagctt tcttgtacaa agtggtgatc ctctcgagcc tctagaacta
     7321 tagtgagtcg tattacgtag atccagacat gataagatac attgatgagt ttggacaaac
     7381 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     7441 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     7501 gtttcaggtt cagggggagg tgtgggaggt tttttaattc gcggccgcgg cgccaatgca
     7561 ttgggcccgg tacccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
     7621 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     7681 cgagccggga gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     7741 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     7801 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     7861 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7921 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7981 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     8041 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     8101 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     8161 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     8221 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     8281 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     8341 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     8401 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     8461 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     8521 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8581 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8641 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     8701 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     8761 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     8821 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8881 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     8941 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     9001 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     9061 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     9121 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     9181 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     9241 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     9301 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     9361 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     9421 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     9481 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     9541 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     9601 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     9661 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     9721 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa
     9781 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     9841 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     9901 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     9961 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
    10021 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
    10081 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
    10141 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
    10201 ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact
    10261 gttgg
//
