LOCUS       sequence-365640-        9086 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tier-3 vector for lentiviral stable integration of the rtTA and 
            puromyine - Ypet selection cassette in inverted direction with polyA
            (PRPBSA-YPet-p2a-PuroR::PPGK-rtTA-pA::A3-pA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9086)
  AUTHORS   Haellman V, Strittmatter T, Bertschi A, Stucheli P, Fussenegger M
  TITLE     A versatile plasmid architecture for mammalian synthetic biology 
            (VAMSyB).
  JOURNAL   Metab Eng. 2021 Jul;66:41-50. doi: 10.1016/j.ymben.2021.04.003. Epub
            2021 Apr 18.
  PUBMED    33857582
REFERENCE   2  (bases 1 to 9086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2021-07"; volume: "66:41-50. doi"; pages: " 
            10.1016/j.ymben.2021.04.003. Epub 2021 Apr 18"
COMMENT     Sequence Label: pTS2334_Tier3(Lenti_inverse)-rtTA-YPet_Puro
FEATURES             Location/Qualifiers
     source          1..9086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      257..845
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     746..765
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             908..1141
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    1213..1801
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1319..1330
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1728..1748
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     complement(1975..1992)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1981..2205
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        2253..2752
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2288..2311)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2685..2704
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2805..3545
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="SRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHVK
                     NKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTRP
                     TEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTTD
                     SMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPAD
                     ALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(3667..3683)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3667..3683)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4676..4695)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             5114..5170
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5189..5782
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     5682..5702
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5966..6083
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6381..6425)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6610..6843
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7336..7461
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7508..7688
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7706..7904)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7731..7751)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7906..8209
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8257..8361
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8362..8430,8431..9086,1..136)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8580..8599)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
       61 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
      121 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
      181 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc cccgtagaaa
      241 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      301 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      361 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      421 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      481 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      541 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      601 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      661 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      721 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      781 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      841 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      901 acatgtctgc tagagatttt ccacactgac taaaagggtc tgagggatct ctagttacca
      961 gagtcacaca acagacgggc acacactact tgaagcactc aaggcaaact ttattgaggc
     1021 ttaagcagtg ggttccctag ttagccagag agctcccagg ctcagatctg gtctaaccag
     1081 agaaacccag tacaagcaaa aagcagatct tgtcttcgtt gggagtgaat tagcccttcc
     1141 agtcccccct tttcttttaa aaagtggcta agatctacag ctgccttgta agtcattggt
     1201 cttaaagtcg acgcggggag gcggcccaaa gggagatccg actcgtctga gggcgaaggc
     1261 gaagacgcgg aagaggccgc agagccggca gcaggccgcg ggaaggaagg tccgctggat
     1321 tgagggccga agggacgtag cagaaggacg tcccgcgcag aatccaggtg gcaacacagg
     1381 cgagcagcca aggaaaggac gatgatttcc ccgacaacac cacggaattg tcagtgccca
     1441 acagccgagc ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc gccgtggcaa
     1501 tagggagggg gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca atgccccaac
     1561 cagtgggggt tgcgtcagca aacacagtgc acaccacgcc acgttgcctg acaacgggcc
     1621 acaactcctc ataaagagac agcaaccagg atttatacaa ggaggagaaa atgaaagcca
     1681 tacgggaagc aatagcatga tacaaaggca ttaaagcagc gtatccacat agcgtaaaag
     1741 gagcaacata gttaagaata ccagtcaatc tttcacaaat tttgtaatcc agaggttgat
     1801 tgatctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata
     1861 gctacgcgtg gtaccctcga ggagctcttc gaagcggaat tcaccatgac tagtgctagc
     1921 ggatccaccg gtgtctagaa agctttgagg ccggcctgca ggcccgctga tcagcctcga
     1981 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     2041 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     2101 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     2161 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggtagct taggaggcgg
     2221 aaagaaccag cgcgtggtac cctcgagcta ccgggtaggg gaggcgcttt tcccaaggca
     2281 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
     2341 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
     2401 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
     2461 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcagt agtctcgtgc agatggacag
     2521 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
     2581 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
     2641 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
     2701 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctccag
     2761 cccgagctcg gtatagggag accgaattca ccatgactag tggatcaaga ctggacaaga
     2821 gcaaagtcat aaactctgct ctggaattac tcaatggagt cggtatcgaa ggcctgacga
     2881 caaggaaact cgctcaaaag ctgggagttg agcagcctac cctgtactgg cacgtgaaga
     2941 acaagcgggc cctgctcgat gccctgccaa tcgagatgct ggacaggcat catacccact
     3001 cctgccccct ggaaggcgag tcatggcaag actttctgcg gaacaacgcc aagtcatacc
     3061 gctgtgctct cctctcacat cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag
     3121 agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc
     3181 tggagaacgc actgtacgct ctgtccgccg tgggccactt tacactgggc tgcgtattgg
     3241 aggaacagga gcatcaagta gcaaaagagg aaagagagac acctaccacc gattctatgc
     3301 ccccacttct gaaacaagca attgagctgt tcgaccggca gggagccgaa cctgccttcc
     3361 ttttcggcct ggaactaatc atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg
     3421 ggccgaccga cgcccttgac gattttgact tagacatgct cccagccgat gcccttgacg
     3481 actttgacct tgatatgctg cctgctgacg ctcttgacga ttttgacctt gacatgctcc
     3541 ccggggctag cggatccacc ggtgtctaga aagctataca ggtgcaccgc tttataataa
     3601 aactgataca gagaacgtta tcatgtgtgt tggttttttt tgtgtgtgcg gtttaaggga
     3661 gcctcggtca tagctgtttc ctgtgtaaaa cgacggccag cgcgtggtac cctcgagcgt
     3721 gaccctgtct cacaaaataa agtaagcccg gactgagtgc ggaaaggcgg gcctggcggg
     3781 tctggactcc ccatgcgggc caccagaggc cctgcagcct tcagtcgctt gaaggggtaa
     3841 tggcgcttcc actcacaaac atggcggaca gagcgtgtga acgagatgaa cagcccctca
     3901 aaaatatggc cgccgaggct ggacggccgt gccccagcag caccgcctcc gcgccccacg
     3961 tgatctctcg ccgggcacag cgctgaccgc ggaggtccaa ccggaagaat gtccggattg
     4021 gacattcgga agagggcccg ccttccctgg ggaatctctg cgcacgcgca gaacgcttcg
     4081 accaatgaaa acacaggaag ccgtccgcgc aaccgcgttg cgtcacttct gccgcccctg
     4141 tttcaaggta tatagccgta gacggaactt cgcctttctc tcggccttag cgccattttt
     4201 ttgggtgagt gttttttggt tcctgcgttg ggattccgtg tacaatccat agacatctga
     4261 cctcggcact tagcatcatc acagcaaact aactgtagcc tttctctctt tccctgtaga
     4321 aacctctgca cctagggagc tcgaattcac catgactagt ggtggttctg gtgtgagcaa
     4381 aggcgaagag ctgttcaccg gcgtggtgcc catcctggtg gagctggacg gcgacgtgaa
     4441 cggccacaag ttcagcgtga gcggcgaggg cgagggcgac gccacctacg gcaagctgac
     4501 cctgaagctg ctgtgcacca ccggcaagct gcccgtgccc tggcccaccc tggtgaccac
     4561 cctgggctac ggcgtgcagt gcttcgcccg gtaccccgac cacatgaagc agcacgactt
     4621 cttcaagagc gccatgcccg agggctacgt gcaggagcgg accatcttct tcaaggacga
     4681 cggcaactac aagacccggg ccgaggtgaa gttcgagggc gacaccctgg tgaaccggat
     4741 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctgggccaca agctggagta
     4801 caactacaac agccacaacg tgtacatcac cgccgacaag cagaagaacg gcatcaaggc
     4861 caacttcaag atccggcaca acatcgagga cggcggcgtg cagctggccg accactacca
     4921 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta
     4981 ccagagcgcc ctgttcaagg accccaacga gaagcgggac cacatggtgc tgctggagtt
     5041 cctgaccgcc gccggcatca ccgagggcat ggacgagctg tacaaggcta gtggtggttc
     5101 tggtggaagc ggagctacta acttcagcct gctgaagcag gctggtgacg tggaggagaa
     5161 ccctggacct gctagtggtg gttctggtac cgagtacaag cccacggtgc gcctcgccac
     5221 ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     5281 cacgcgccac accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     5341 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     5401 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     5461 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     5521 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtgtcgcc
     5581 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     5641 gcgcgccggg gtgcccgcct tcctggagac atccgcgccc cgcaacctcc ccttctacga
     5701 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     5761 catgacccgc aagcccggtg ccgctagcgg atccaccggt gtctagaaag ctcagaaatt
     5821 atgagagcgt ttaataaagt attgactatg gttgacaaaa cgtgtgttgg agttttttgt
     5881 gtgctttgtc atcaactgag ccagtagcgc gtgaccaagt atccccgctt cttctcgcgt
     5941 cactgctgtc cctgtaataa acccgaaaat tttgaatttt tgtaatttgt ttttgtaatt
     6001 ctttagtttg tatgtctgtt gctattatgt ctactattct ttcccctgca ctgtaccccc
     6061 caatcccccc ttttctttta aaattgtgga tgaatactgc catttgtctg cagaattggc
     6121 gcacgcagtg ccgatccgtt cactaatcga atggatctgt ctctgtctct ctctccacct
     6181 tcttcttcta ttccttcggg cctgtcgggt cccctcgggg ttgggaggtg ggtctgaaac
     6241 gataatggtg aatatccctg cctaactcta ttcactatag aaagtacagc aaaaactatt
     6301 cttaaaccta ccaagcctcc tactatcatt atgaataatt ttatatacca cagccaattt
     6361 gttatgttaa accaattcca caaacttgcc catttatcta attccaataa ttcttgttca
     6421 ttcttttctt gctggttttg cgattcttca attaaggagt gtattaagct tgtgtaattg
     6481 ttaatttctc tgtcccactc catccaggtc gtgtgattcc aaatctgttc cagagattta
     6541 ttactccaac tagcattcca aggcacagca gtggtgcaaa tgagttttcc agagcaaccc
     6601 caaatcccca ggagctgttg atcctttagg tatctttcca cagccaggat tcttgcctgg
     6661 agctgcttga tgccccagac tgtgagttgc aacagatgct gttgcgcctc aatagccctc
     6721 agcaaattgt tctgctgctg cactatacca gacaataatt gtctggcctg taccgtcagc
     6781 gtcattgacg ctgcgcccat agtgcttcct gctgctccca agaacccaag gaacaaagct
     6841 cctattccca ctgctctttt ttctctctgc accactcttc tctttgcctt ggtgggtgct
     6901 actcctaatg gttcaatttt tactacttta tatttatata attcacttct ccaattgtcc
     6961 ctcatatctc ctcctccagg tctgaagatc agcggccgct tgctgtgcgg tggtcttact
     7021 tttgttttgc tcttcctcta tcttgtctaa agcttccttg gtgtctttta tctctatcct
     7081 ttgatgcaca caatagaggg ttgctactgt attatataat gatctaagtt cttctgatcc
     7141 tgtctgaagg gatggttgta gctgtcccag tatttgtcta cagccttctg atgtttctaa
     7201 caggccagga ttaactgcga atcgttctag ctccctgctt gcccatacta tatgttttaa
     7261 tttatatttt ttctttcccc ctggccttaa ccgaattttt tcccatcgcg atctaattct
     7321 cccccgctta atactgacgc tctcgcaccc atctctctcc ttctagcctc cgctagtcaa
     7381 aatttttggc gtactcacca gtcgccgccc ctcgcctctt gccgtgcgcg cttcagcaag
     7441 ccgagtcctg cgtcgagaga gctcctctgg tttccctttc gctttcaagt ccctgttcgg
     7501 gcgccactgc tagagatttt ccacactgac taaaagggtc tgagggatct ctagttacca
     7561 gagtcacaca acagacgggc acacactact tgaagcactc aaggcaaact ttattgaggc
     7621 ttaagcagtg ggttccctag ttagccagag agctcccagg ctcagatctg gtctaaccag
     7681 agaaacccag tacaggcaaa acgcgctgct tatatagacc tcccaccgta cacgcctacc
     7741 gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc gttgattttg
     7801 gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc
     7861 aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg taatagcgat
     7921 gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata
     7981 atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata
     8041 cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg
     8101 gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca atgggcgggg
     8161 gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacga gatcttggtt
     8221 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     8281 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     8341 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     8401 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     8461 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     8521 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     8581 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     8641 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     8701 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     8761 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     8821 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     8881 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     8941 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     9001 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     9061 ggagccggtg agcgtgggag ccgcgg
//
