LOCUS       sequence-365653-         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PCR template for repair templates to tag a gene with a GFP and mIAA7
            AID degron using homology-directed repair for C. elegans..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Sepers JJ, Verstappen NHM, Vo AA, Ragle JM, Ruijtenberg S, Ward JD, 
            Boxem M
  TITLE     The mIAA7 degron improves auxin-mediated degradation in C. elegans.
  JOURNAL   G3 (Bethesda). 2022 Aug 27. pii: 6677660. doi: 
            10.1093/g3journal/jkac222.
  PUBMED    36029236
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Aug 27. pii: 6677660. doi: 
            10.1093/g3journal/jkac222."
COMMENT     Sequence Label: pJJS002
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..303,357..431,483..602,654..869)
                     /codon_start=1
                     /product="monomeric enhanced GFP, optimized with synthetic 
                     introns for expression in C. elegans"
                     /label=mEGFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLKE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 atgtccaagg gagaggaact cttcaccgga gtcgtcccaa tcctcgtcga gctcgacgga
       61 gacgtcaacg gacacaagtt ctccgtctca ggagagggag agggagacgc cacctacgga
      121 aagctcaccc tcaagttcat ctgcaccacc ggaaagctcc cagtcccatg gccaaccctc
      181 gtcaccacct tcacttacgg agtccaatgc ttctcccgtt acccagacca catgaagcgt
      241 cacgacttct tcaagtccgc catgccagag ggatacgtcc aagagcgtac catcttcttc
      301 aaggtaagtt taaacattaa ttaatactaa ctaaccctga ttatttaaat tttcaggacg
      361 acggaaacta caagacccgt gccgaggtca agttcgaggg agacaccctc gtcaaccgta
      421 tcgagctcaa ggtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc
      481 agggaatcga cttcaaggag gacggaaaca tcctcggaca caagctcgaa tacaactaca
      541 actcccacaa cgtctacatc atggccgaca agcaaaagaa cggaatcaag gtcaacttca
      601 aggtaagttt aaacatgatt ttactaacta actaatctga tttaaatttt cagatccgtc
      661 acaacatcga ggacggatct gtccaactcg ccgaccacta ccaacaaaac accccaatcg
      721 gagacggacc agtcctcctc ccagacaacc actacctctc cacccaatcc gccctctcca
      781 aggacccaaa cgagaagcgt gaccacatgg tcctcaagga gttcgtcacc gctgccggaa
      841 tcacccacgg aatggacgag ctctacaag
//
