LOCUS       sequence-365656-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PCR template for repair templates to tag a gene with a mCherry and 
            mIAA7 AID degron using homology-directed repair for C. elegans..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Sepers JJ, Verstappen NHM, Vo AA, Ragle JM, Ruijtenberg S, Ward JD, 
            Boxem M
  TITLE     The mIAA7 degron improves auxin-mediated degradation in C. elegans.
  JOURNAL   G3 (Bethesda). 2022 Aug 27. pii: 6677660. doi: 
            10.1093/g3journal/jkac222.
  PUBMED    36029236
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Aug 27. pii: 6677660. doi: 
            10.1093/g3journal/jkac222."
COMMENT     Sequence Label: pJJS003
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgtccaagg gagaggagga caacatggcc atcatcaagg agttcatgcg tttcaaggtc
       61 cacatggagg gatcagtcaa cggacacgag ttcgagatcg agggagaggg agagggacgt
      121 ccatacgagg gaacccaaac cgccaagctc aaggtaagtt taaacatata tatactaact
      181 aaccctgatt atttaaattt tcaggtcacc aagggaggac cactcccatt cgcctgggac
      241 atcctctccc cacaattcat gtacggatca aaggcctacg tcaagcaccc agccgacatc
      301 ccagactacc tcaagctctc cttcccagag ggattcaagt gggagcgtgt catgaacttc
      361 gaggacggag gagtcgtcac cgtcacccaa gactcctccc tccaagacgg agagttcatc
      421 tacaaggtaa gtttaaacag ttcggtacta actaaccata catatttaaa ttttcaggtc
      481 aagctccgtg gaaccaactt cccatccgac ggaccagtca tgcaaaagaa gaccatggga
      541 tgggaggcct cctccgagcg tatgtaccca gaggacggag ccctcaaggg agagatcaag
      601 caacgtctca agctcaagga cggaggacac tacgacgccg aggtcaagac cacctacaag
      661 gccaagaagc cagtccaact cccaggtaag tttaaacatg attttactaa ctaactaatc
      721 tgatttaaat tttcaggagc ctacaacgtc aacatcaagc tcgacatcac ctcccacaac
      781 gaggactaca ccatcgtcga gcaatacgag cgtgccgagg gacgtcactc caccggagga
      841 atggacgagc tctacaag
//
