LOCUS       sequence-365668-         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PCR template for repair templates to tag a gene with a wrmScarlet 
            and mIAA7 AID degron using homology-directed repair for C. elegans..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Sepers JJ, Verstappen NHM, Vo AA, Ragle JM, Ruijtenberg S, Ward JD, 
            Boxem M
  TITLE     The mIAA7 degron improves auxin-mediated degradation in C. elegans.
  JOURNAL   G3 (Bethesda). 2022 Aug 27. pii: 6677660. doi: 
            10.1093/g3journal/jkac222.
  PUBMED    36029236
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Aug 27. pii: 6677660. doi: 
            10.1093/g3journal/jkac222."
COMMENT     Sequence Label: pJJS007
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggtcagca agggagaggc agttatcaag gagttcatgc gtttcaaggt ccacatggag
       61 ggatccatga acggacacga gttcgagatc gagggagagg gagagggacg tccatacgag
      121 ggaacccaaa ccgccaagct caaggtcacc aaggtaagtt taaacatata tatactaact
      181 aaccctgatt atttaaattt tcagggagga ccactcccat tctcctggga catcctctcc
      241 ccacaattca tgtacggatc ccgtgccttc accaagcacc cagccgacat cccagactac
      301 tacaagcaat ccttcccaga gggattcaag tgggagcgtg tcatgaactt cgaggacgga
      361 ggagccgtca ccgtcaccca agacacctcc ctcgaggacg gaaccctcat ctacaaggta
      421 agtttaaaca gttcggtact aactaaccat acatatttaa attttcaggt caagctccgt
      481 ggaaccaact tcccaccaga cggaccagtc atgcaaaaga agaccatggg atgggaggcc
      541 tccaccgagc gtctctaccc agaggacgga gtcctcaagg gagacatcaa gatggccctc
      601 cgtctcaagg acggaggacg ttacctcgcc gacttcaagg taagtttaaa catgatttta
      661 ctaactaact aatctgattt aaattttcag accacctaca aggccaagaa gccagtccaa
      721 atgccaggag cctacaacgt cgaccgtaag ctcgacatca cctcccacaa cgaggactac
      781 accgtcgtcg agcaatacga gcgttccgag ggacgtcact ccaccggagg aatggacgag
      841 ctctacaag
//
