LOCUS       sequence-365674-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PCR template for repair templates to tag a gene with a mKate2 and 
            mIAA7 AID degron using homology-directed repair for C. elegans..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Sepers JJ, Verstappen NHM, Vo AA, Ragle JM, Ruijtenberg S, Ward JD, 
            Boxem M
  TITLE     The mIAA7 degron improves auxin-mediated degradation in C. elegans.
  JOURNAL   G3 (Bethesda). 2022 Aug 27. pii: 6677660. doi: 
            10.1093/g3journal/jkac222.
  PUBMED    36029236
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Aug 27. pii: 6677660. doi: 
            10.1093/g3journal/jkac222."
COMMENT     Sequence Label: pJJS009
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggtctccg agctcattaa agaaaacatg catatgaagc tctacatgga gggaaccgtc
       61 aacaaccacc acttcaagtg cacctccgag ggagagggaa agccatacga gggaacccaa
      121 accatgcgta tcaaggccgt cgagggagga ccactcccat tcgccttcga catcctcgcc
      181 acctccttca tgtacggatc caaggtaagt ttaaacatat atatactaac taaccctgat
      241 tatttaaatt ttcagacctt catcaaccac acccaaggaa tcccagactt cttcaagcaa
      301 tccttcccag agggattcac ctgggagcgt gtcaccacct acgaggacgg aggagtcctc
      361 accgccaccc aagacacctc cctccaagac ggatgcctca tctacaacgt caaggtaagt
      421 ttaaacagtt cggtactaac taaccataca tatttaaatt ttcagatccg tggagtcaac
      481 ttcccatcca acggaccagt catgcaaaag aagaccctcg gatgggaggc ctccaccgag
      541 accctctacc cagccgacgg aggacttgag ggacgtgccg acatggccct caagctcgtc
      601 ggaggaggac acctcatctg caacctcaag gtaagtttaa acatgatttt actaactaac
      661 taatctgatt taaattttca gaccacctac cgttccaaga agccagccaa gaacctcaag
      721 atgccaggag tctactacgt cgaccgtcgt cttgagcgta tcaaggaggc cgacaaggag
      781 acctacgtcg agcaacacga ggtcgccgtc gcccgttact gcgacctccc atccaagctc
      841 ggacaccgta ag
//
