LOCUS       sequence-365680-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PCR template for repair templates to tag a gene with a mTagBFP2 and 
            mIAA7 AID degron using homology-directed repair for C. elegans..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Sepers JJ, Verstappen NHM, Vo AA, Ragle JM, Ruijtenberg S, Ward JD, 
            Boxem M
  TITLE     The mIAA7 degron improves auxin-mediated degradation in C. elegans.
  JOURNAL   G3 (Bethesda). 2022 Aug 27. pii: 6677660. doi: 
            10.1093/g3journal/jkac222.
  PUBMED    36029236
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Aug 27. pii: 6677660. doi: 
            10.1093/g3journal/jkac222."
COMMENT     Sequence Label: pJJS011
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggtttcgg aattgattaa ggaaaatatg cacatgaagc tctacatgga gggaaccgtc
       61 gacaaccacc acttcaagtg cacctccgag ggagagggaa agccatacga gggaacccaa
      121 accatgcgta tcaaggtcgt cgagggagga ccactcccat tcgccttcga catcctcgcc
      181 acctccttcc tctacggatc caaggtaagt ttaaacatat atatactaac taaccctgat
      241 tatttaaatt ttcagacctt catcaaccac acccaaggaa tcccagactt cttcaagcaa
      301 tccttcccag agggattcac ctgggagcgt gtcaccacct acgaggacgg aggagtcctc
      361 accgccaccc aagacacctc cctccaagac ggatgcctca tctacaacgt caaggtaagt
      421 ttaaacagtt cggtactaac taaccataca tatttaaatt ttcagatccg tggagtcaac
      481 ttcacctcca acggaccagt catgcaaaag aagaccctcg gatgggaggc cttcaccgag
      541 accctctacc cagccgacgg aggactcgag ggacgtaacg acatggccct caagctcgtc
      601 ggaggatccc acctcatcgc caacgccaag gtaagtttaa acatgatttt actaactaac
      661 taatctgatt taaattttca gaccacctac cgttccaaga agccagccaa gaacctcaag
      721 atgccaggag tctactacgt cgactaccgt ctcgagcgta tcaaggaggc caacaacgag
      781 acctacgtcg agcaacacga ggtcgccgtc gcccgttact gcgacctccc atccaagctc
      841 ggacacaagc tcaac
//
