LOCUS       sequence-365698-        6205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV vector packaging QPAS1-VP16, a component of optogenetic system 
            for NIR light-controllable transcriptional activation .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6205)
  AUTHORS   Kasatkina LA, Ma C, Matlashov ME, Vu T, Li M, Kaberniuk AA, Yao J, 
            Verkhusha VV
  TITLE     Optogenetic manipulation and photoacoustic imaging using a 
            near-infrared transgenic mouse model.
  JOURNAL   Nat Commun. 2022 May 19;13(1):2813. doi: 10.1038/s41467-022-30547-6.
  PUBMED    35589810
REFERENCE   2  (bases 1 to 6205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-05-19"; volume: "13(1):2813. doi"; pages: " 
            10.1038/s41467-022-30547-6"
COMMENT     Sequence Label: pAAV-CAG-NLS-QPAS1-VP16
FEATURES             Location/Qualifiers
     source          1..6205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     465..487
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     549..568
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1133..1147
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     regulatory      1277..1286
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1436..1669
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     misc_feature    1697..2285
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1750..1770)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2168..2179)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2317..2793
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2516..2535)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   2833..2973
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2833..2962
                     /label=AAV2 ITR
     rep_origin      3048..3503
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3135..3154)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3345..3366
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3520..3539)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3639..3661
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3699..3717)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3785..3889
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3890..3958,3959..4750)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4108..4127)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4921..5509
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5410..5429
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   5571..5700
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     enhancer        5780..6083
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(6085..6205,1..135)
                     /label=chicken beta-actin promoter
                     /note="contains a 21-bp deletion relative to the standard 
                     chicken beta-actin promoter"
ORIGIN
        1 cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc
       61 gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga
      121 agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc
      181 gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg
      241 gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt
      301 ctgtggctgc gtgaaagcct tgaggggctc cgggagcgcc ggcaggaagg aaatgggcgg
      361 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
      421 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
      481 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
      541 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattgcggcc
      601 caacggtacc gccatgtcgc ggaggcggca cagctacgag aacgatggtg gacaacctca
      661 caaaaggagg aagacgtctc caccgggcaa gaacatgcag gcggtcaccg agctgcattc
      721 ccggctgatc gctgcgcagc aggcgatgga gcgcgactat tggcggttgc gtgaattgga
      781 gactcgctac cgcctggtgt tcgacgctgc cgccgatgcg gtgatgatcg tctccgccgg
      841 cgacatgcgc atcgtcgaag ccaaccgggc ggcggtgaat gcgatcagcc gcgtcgagcg
      901 cggcaatgac gaccttgcgg ggcgtgattt cctcgccgaa gtggcggctg ccgatcgcga
      961 tgcggtgcgc gacatgctgg cccaggtgcg tcagcgcggc accgcactca gcgtcctcgt
     1021 tcatctcggc cgttacgacc gcgcctggat gctgcgcggt tcgctgatgt cgtccgagcg
     1081 tcgtcaggtt ttcctgctgc acttcacccc ggtgaccacg actcccgcga tcgacgacga
     1141 cgacaaaggt gtcgttgctt ctgctgccga cggtgcagag ggcgcctcag acgacgcaga
     1201 ggataagctt agtgctggtg gtagtgctgg tggtagtgct ggtggtagtg ctggtggtag
     1261 tgctggtggt ccggacgcca ccatggcgta cagccgcgcg cgtacgaaaa acaattacgg
     1321 gtctaccatc gagggcctgc tcgatctccc ggacgacgac gcccccgaag aggcggggct
     1381 ggcggctccg cgcctgtcct ttctccccgc gggacacacg cgcagactgt cgacggcccc
     1441 cccgaccgat gtcagcctgg gggacgagct ccacttagac ggcgaggacg tggcgatggc
     1501 gcatgccgac gcgctagacg atttcgatct ggacatgttg ggggacgggg attccccggg
     1561 tccgggattt accccccacg actccgcccc ctacggcgct ctggatatgg ccgacttcga
     1621 gtttgagcag atgtttaccg atgcccttgg aattgacgag tacggtgggt aagaattcga
     1681 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     1741 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     1801 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     1861 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     1921 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     1981 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     2041 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     2101 ctttccttgg ctgctcgcct atgttgccac ctggattctg cgcgggacgt ccttctgcta
     2161 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     2221 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     2281 cccgcatcga taccgagcgc tgctcgagag atctacgggt ggcatccctg tgacccctcc
     2341 ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata
     2401 aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg
     2461 ggggtggtat ggagcaaggg gcaagttggg aagacaacct gtagggcctg cggggtctat
     2521 tgggaaccaa gctggagtgc agtggcacaa tcttggctca ctgcaatctc cgcctcctgg
     2581 gttcaagcga ttctcctgcc tcagcctccc gagttgttgg gattccaggc atgcatgacc
     2641 aggctcagct aatttttgtt tttttggtag agacggggtt tcaccatatt ggccaggctg
     2701 gtctccaact cctaatctca ggtgatctac ccaccttggc ctcccaaatt gctgggatta
     2761 caggcgtgaa ccactgctcc cttccctgtc cttctgattt tgtaggtaac cacgtgcgga
     2821 ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
     2881 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
     2941 cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta
     3001 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag
     3061 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     3121 cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     3181 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     3241 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
     3301 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     3361 aactggaaca acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc
     3421 gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     3481 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc
     3541 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     3601 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     3661 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     3721 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     3781 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3841 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     3901 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     3961 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     4021 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     4081 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     4141 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     4201 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     4261 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     4321 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     4381 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     4441 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     4501 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     4561 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     4621 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     4681 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     4741 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     4801 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     4861 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     4921 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4981 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     5041 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     5101 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     5161 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     5221 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     5281 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     5341 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     5401 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     5461 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     5521 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt cctgcaggca
     5581 gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt ggtcgcccgg
     5641 cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact aggggttcct
     5701 gcggccgcac gcgtaacact agttattaat agtaatcaat tacggggtca ttagttcata
     5761 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     5821 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     5881 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     5941 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     6001 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     6061 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
     6121 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
     6181 cgatgggggc gggggggggg sgggg
//
