LOCUS       sequence-365703-        1922 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sub-array spacer 2/4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Shaw WM, Studena L, Roy K, Hapeta P, McCarty NS, Graham AE, Ellis T,
            Ledesma-Amaro R
  TITLE     Inducible expression of large gRNA arrays for multiplexed CRISPRai 
            applications.
  JOURNAL   Nat Commun. 2022 Aug 25;13(1):4984. doi: 10.1038/s41467-022-32603-7.
  PUBMED    36008396
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-08-25"; volume: "13(1):4984. doi"; pages: " 
            10.1038/s41467-022-32603-7"
COMMENT     Sequence Label: pWS3806
FEATURES             Location/Qualifiers
     source          1..1922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(167..958,959..1027))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     790..809
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1028..1132)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1235..1823
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1724..1743
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgtctcttgc cccataaacg tgctattagt tattaggtcc gaagattgaa aaaggtgagg
       61 gctaaagaga cggattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
      121 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
      181 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
      241 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
      301 taccgcggga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
      361 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
      421 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
      481 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
      541 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
      601 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
      661 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
      721 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
      781 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
      841 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
      901 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
      961 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     1021 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     1081 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     1141 cccgaaaagt gccacctgtc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1201 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1261 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1321 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1381 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     1441 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     1501 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     1561 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     1621 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     1681 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     1741 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     1801 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     1861 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     1921 gt
//
