LOCUS       sequence-365711-       11310 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of GFP and botulinum neurotoxin type B light 
            chain from UbC promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11310)
  AUTHORS   Albarran E, Sun Y, Liu Y, Raju K, Dong A, Li Y, Wang S, Sudhof TC, 
            Ding JB
  TITLE     Postsynaptic synucleins mediate endocannabinoid signaling.
  JOURNAL   Nat Neurosci. 2023 Jun;26(6):997-1007. doi: 
            10.1038/s41593-023-01345-0. Epub 2023 May 29.
  PUBMED    37248337
REFERENCE   2  (bases 1 to 11310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41593-023-01345-0"; journalName: "Nat Neurosci"; date: 
            "2023-06"; volume: "26"; issue: "6"; pages: "997-1007"
COMMENT     Sequence Label: FUGW-UBC-EGFP-2A-BoNT-B (LC)
FEATURES             Location/Qualifiers
     source          1..11310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        191..570
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        572..770
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     725..745
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             788..968
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1015..1140
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1633..1866
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2051..2095
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2393..2510
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2575..3786
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3623..3642
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3834..3843
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3840..3842,3843..3845,3846..4556)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3840..3842,3843..3845,3846..4556)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3885..3906)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4146..4165)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4493..4514
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4578..4643
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     misc_feature    6000..6588
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6053..6073)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6471..6482)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6591..6607)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6592..6608)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7113..7293
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7319..7336)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7325..7549
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7595..8023
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7682..7701)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7892..7913
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8032..8052)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8037..8366
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8217..8352
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8279..8298
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8414..8461
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8480..8854
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8984..9105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9021..9040)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9075..9094
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9154..9170)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9154..9170)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9167..9189)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9178..9194
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9202..9208,9209..9226,9227..9232))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9247..9268
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9385..9402)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9556..10144)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9636..9655)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10315..11106,11107..11175))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10938..10957
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11176..11280)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(11310,1..19))
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
       61 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
      121 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
      181 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
      241 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
      301 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      361 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      421 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      481 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      541 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      601 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      661 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      721 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc
      781 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
      841 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
      901 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
      961 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     1021 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     1081 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     1141 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     1201 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     1261 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     1321 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     1381 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     1441 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     1501 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     1561 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     1621 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     1681 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     1741 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     1801 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     1861 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     1921 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     1981 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     2041 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     2101 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     2161 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     2221 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     2281 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     2341 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
     2401 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     2461 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     2521 acagggacag cagagatcca gtttggttaa ttaacccgtg tcggctccag atctggcctc
     2581 cgcgccgggt tttggcgcct cccgcgggcg cccccctcct cacggcgagc gctgccacgt
     2641 cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg acagcggccc
     2701 gctgctcata agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg
     2761 acttgggtga ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt
     2821 agtcccttct cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgattat
     2881 ataaggacgc gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt
     2941 tcttgtttgt ggatcgctgt gatcgtcact tggtgagtag cgggctgctg ggctggccgg
     3001 ggctttcgtg gccgccgggc cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg
     3061 ctgtagtctg ggtccgcgag caaggttgcc ctgaactggg ggttgggggg agcgcagcaa
     3121 aatggcggct gttcccgagt cttgaatgga agacgcttgt gaggcgggct gtgaggtcgt
     3181 tgaaacaagg tggggggcat ggtgggcggc aagaacccaa ggtcttgagg ccttcgctaa
     3241 tgcgggaaag ctcttattcg ggtgagatgg gctggggcac catctgggga ccctgacgtg
     3301 aagtttgtca ctgactggag aactcggttt gtcgtctgtt gcgggggcgg cagttatggc
     3361 ggtgccgttg ggcagtgcac ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg
     3421 tcacccgttc tgttggctta taatgcaggg tggggccacc tgccggtagg tgtgcggtag
     3481 gcttttctcc gtcgcaggac gcagggttcg ggcctagggt aggctctcct gaatcgacag
     3541 gcgccggacc tctggtgagg ggagggataa gtgaggcgtc agtttctttg gtcggtttta
     3601 tgtacctatc ttcttaagta gctgaagctc cggttttgaa ctatgcgctc ggggttggcg
     3661 agtgtgtttt gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt
     3721 aattttcagt gttagactag taaattgtcc gctaaattct ggccgttttt ggcttttttg
     3781 ttagacgaag cttgggctgc aggtcgactc tagaggatcc ccgggtaccg gtcgccacca
     3841 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     3901 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     3961 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     4021 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     4081 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     4141 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     4201 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     4261 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     4321 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     4381 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     4441 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     4501 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaaggcgc
     4561 gccaaattgt cgctcctgtc aaacaaactc ttaactttga tttactcaaa ctggctgggg
     4621 atgtagaaag caatccaggt ccagctagca tgccagttac aataaataat tttaattata
     4681 atgatcctat tgataataat aatattatta tgatggagcc tccatttgcg agaggtacgg
     4741 ggagatatta taaagctttt aaaatcacag atcgtatttg gataataccg gaaagatata
     4801 cttttggata taaacctgag gattttaata aaagttccgg tatttttaat agagatgttt
     4861 gtgaatatta tgatccagat tacttaaata ctaatgataa aaagaatata tttttacaaa
     4921 caatgatcaa gttatttaat agaatcaaat caaaaccatt gggtgaaaag ttattagaga
     4981 tgattataaa tggtatacct tatcttggag atagacgtgt tccactcgaa gagtttaaca
     5041 caaacattgc tagtgtaact gttaataaat taatcagtaa tccaggagaa gtggagcgaa
     5101 aaaaaggtat tttcgcaaat ttaataatat ttggacctgg gccagtttta aatgaaaatg
     5161 agactataga tataggtata caaaatcatt ttgcatcaag ggaaggcttc gggggtataa
     5221 tgcaaatgaa gttttgccca gaatatgtaa gcgtatttaa taatgttcaa gaaaacaaag
     5281 gcgcaagtat atttaataga cgtggatatt tttcagatcc agccttgata ttaatgcatg
     5341 aacttataca tgttttacat ggattatatg gcattaaagt agatgattta ccaattgtac
     5401 caaatgaaaa aaaatttttt atgcaatcta cagatgctat acaggcagaa gaactatata
     5461 catttggagg acaagatccc agcatcataa ctccttctac ggataaaagt atctatgata
     5521 aagttttgca aaattttaga gggatagttg atagacttaa caaggtttta gtttgcatat
     5581 cagatcctaa cattaatatt aatatatata aaaataaatt taaagataaa tataaattcg
     5641 ttgaagattc tgagggaaaa tatagtatag atgtagaaag ttttgataaa ttatataaaa
     5701 gcttaatgtt tggttttaca gaaactaata tagcagaaaa ttataaaata aaaactagag
     5761 cttcttattt tagtgattcc ttaccaccag taaaaataaa aaatttatta gataatgaaa
     5821 tctatactat agaggaaggg tttaatatat ctgataaaga tatggaaaaa gaatatagag
     5881 gtcagaataa agctataaat aaacaagctt atgaagaaat tagcaaggag catttggctg
     5941 tatataagat acaaatgtgt aaaagtgtta aataagaatt cgatatcaag cttatcgata
     6001 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     6061 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     6121 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     6181 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     6241 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     6301 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     6361 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     6421 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     6481 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     6541 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc
     6601 gacctcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg
     6661 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc
     6721 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     6781 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt
     6841 ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga
     6901 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg
     6961 tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga
     7021 tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc
     7081 acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag
     7141 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     7201 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     7261 gaccctttta gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg ctgatcagcc
     7321 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     7381 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     7441 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     7501 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     7561 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     7621 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     7681 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     7741 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     7801 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     7861 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     7921 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     7981 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     8041 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     8101 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     8161 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     8221 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     8281 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     8341 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     8401 ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga
     8461 caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg
     8521 cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt
     8581 ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca
     8641 ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct
     8701 gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat
     8761 gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa
     8821 ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac
     8881 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     8941 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     9001 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     9061 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     9121 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     9181 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     9241 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     9301 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     9361 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     9421 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     9481 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     9541 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     9601 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     9661 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     9721 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     9781 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     9841 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     9901 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     9961 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
    10021 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
    10081 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    10141 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    10201 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
    10261 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
    10321 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    10381 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    10441 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    10501 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    10561 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    10621 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    10681 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    10741 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    10801 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    10861 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    10921 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    10981 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    11041 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    11101 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    11161 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    11221 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    11281 cacatttccc cgaaaagtgc cacctgacgt
//
