LOCUS       sequence-365713-       10248 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of GFP and dominant-negative VAMP2 from UbC 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10248)
  AUTHORS   Albarran E, Sun Y, Liu Y, Raju K, Dong A, Li Y, Wang S, Sudhof TC, 
            Ding JB
  TITLE     Postsynaptic synucleins mediate endocannabinoid signaling.
  JOURNAL   Nat Neurosci. 2023 Jun;26(6):997-1007. doi: 
            10.1038/s41593-023-01345-0. Epub 2023 May 29.
  PUBMED    37248337
REFERENCE   2  (bases 1 to 10248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41593-023-01345-0"; journalName: "Nat Neurosci"; date: 
            "2023-06"; volume: "26"; issue: "6"; pages: "997-1007"
COMMENT     Sequence Label: FUGW-UBC-EGFP-2A-dnVAMP2
FEATURES             Location/Qualifiers
     source          1..10248
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        191..570
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        572..770
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     725..745
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             788..968
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1015..1140
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1633..1866
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2051..2095
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2393..2510
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2575..3786
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3623..3642
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3834..3843
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3840..3842,3843..3845,3846..4556)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3840..3842,3843..3845,3846..4556)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3885..3906)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4146..4165)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4493..4514
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4566..4622
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    4938..5526
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4991..5011)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5409..5420)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5529..5545)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5530..5546)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6051..6231
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6257..6274)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6263..6487
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6533..6961
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6620..6639)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6830..6851
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6970..6990)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6975..7304
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7155..7290
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7217..7236
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7352..7399
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7418..7792
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7922..8043
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7959..7978)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8013..8032
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8092..8108)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8092..8108)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8105..8127)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8116..8132
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8140..8146,8147..8164,8165..8170))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8185..8206
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8323..8340)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8494..9082)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8574..8593)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9253..10044,10045..10113))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9876..9895
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10114..10218)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(10248,1..19))
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
       61 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
      121 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
      181 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
      241 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
      301 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      361 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      421 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      481 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      541 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      601 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      661 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      721 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc
      781 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
      841 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
      901 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
      961 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     1021 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     1081 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     1141 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     1201 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     1261 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     1321 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     1381 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     1441 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     1501 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     1561 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     1621 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     1681 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     1741 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     1801 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     1861 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     1921 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     1981 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     2041 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     2101 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     2161 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     2221 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     2281 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     2341 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
     2401 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     2461 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     2521 acagggacag cagagatcca gtttggttaa ttaacccgtg tcggctccag atctggcctc
     2581 cgcgccgggt tttggcgcct cccgcgggcg cccccctcct cacggcgagc gctgccacgt
     2641 cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg acagcggccc
     2701 gctgctcata agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg
     2761 acttgggtga ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt
     2821 agtcccttct cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgattat
     2881 ataaggacgc gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt
     2941 tcttgtttgt ggatcgctgt gatcgtcact tggtgagtag cgggctgctg ggctggccgg
     3001 ggctttcgtg gccgccgggc cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg
     3061 ctgtagtctg ggtccgcgag caaggttgcc ctgaactggg ggttgggggg agcgcagcaa
     3121 aatggcggct gttcccgagt cttgaatgga agacgcttgt gaggcgggct gtgaggtcgt
     3181 tgaaacaagg tggggggcat ggtgggcggc aagaacccaa ggtcttgagg ccttcgctaa
     3241 tgcgggaaag ctcttattcg ggtgagatgg gctggggcac catctgggga ccctgacgtg
     3301 aagtttgtca ctgactggag aactcggttt gtcgtctgtt gcgggggcgg cagttatggc
     3361 ggtgccgttg ggcagtgcac ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg
     3421 tcacccgttc tgttggctta taatgcaggg tggggccacc tgccggtagg tgtgcggtag
     3481 gcttttctcc gtcgcaggac gcagggttcg ggcctagggt aggctctcct gaatcgacag
     3541 gcgccggacc tctggtgagg ggagggataa gtgaggcgtc agtttctttg gtcggtttta
     3601 tgtacctatc ttcttaagta gctgaagctc cggttttgaa ctatgcgctc ggggttggcg
     3661 agtgtgtttt gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt
     3721 aattttcagt gttagactag taaattgtcc gctaaattct ggccgttttt ggcttttttg
     3781 ttagacgaag cttgggctgc aggtcgactc tagaggatcc ccgggtaccg gtcgccacca
     3841 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     3901 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     3961 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     4021 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     4081 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     4141 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     4201 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     4261 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     4321 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     4381 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     4441 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     4501 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagggaa
     4561 gcggagccac gaacttctct ctgttaaagc aagcaggaga cgtggaagaa aaccccggtc
     4621 ctatgtctgc taccgctgcc acggcccccc ctgctgcccc ggctggggag ggtggtcccc
     4681 ctgcaccccc tccaaacctc accagtaaca ggagactgca gcagacccag gcccaggtgg
     4741 atgaggtggt ggacatcatg agggtgaacg tggacaaggt cctggagcga gaccagaagc
     4801 tgtcggagct ggacgaccgt gcagatgcac tccaggcggg ggcctcccag tttgaaacaa
     4861 gcgcagccaa gctcaagcgc aaatactggt ggaaaaacct caagatgatg taagaattcg
     4921 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     4981 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     5041 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     5101 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     5161 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     5221 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     5281 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     5341 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     5401 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     5461 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     5521 ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca tggagcaatc acaagtagca
     5581 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
     5641 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     5701 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     5761 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact
     5821 acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac
     5881 cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc
     5941 ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca
     6001 gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct
     6061 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     6121 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     6181 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt
     6241 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     6301 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     6361 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     6421 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     6481 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     6541 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     6601 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     6661 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     6721 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     6781 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     6841 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     6901 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     6961 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     7021 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     7081 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     7141 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     7201 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     7261 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     7321 gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat
     7381 cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt
     7441 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg
     7501 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct
     7561 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt
     7621 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga
     7681 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct
     7741 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct
     7801 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     7861 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     7921 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7981 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     8041 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     8101 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     8161 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     8221 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     8281 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     8341 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     8401 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     8461 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     8521 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     8581 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     8641 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     8701 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     8761 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     8821 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     8881 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     8941 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     9001 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     9061 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     9121 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     9181 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     9241 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     9301 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     9361 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     9421 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     9481 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     9541 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     9601 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     9661 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     9721 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     9781 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     9841 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     9901 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     9961 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    10021 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    10081 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    10141 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    10201 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgt
//
