LOCUS       sequence-365716-       14460 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human Parkin fused to eYFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14460)
  AUTHORS   Tait SW, Oberst A, Quarato G, Milasta S, Haller M, Wang R, Karvela 
            M, Ichim G, Yatim N, Albert ML, Kidd G, Wakefield R, Frase S, 
            Krautwald S, Linkermann A, Green DR
  TITLE     Widespread mitochondrial depletion via mitophagy does not compromise
            necroptosis.
  JOURNAL   Cell Rep. 2013 Nov 27;5(4):878-85. doi: 
            10.1016/j.celrep.2013.10.034. Epub 2013 Nov 21.
  PUBMED    24268776
REFERENCE   2  (bases 1 to 14460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2013.10.034"; journalName: "Cell Rep"; date: 
            "2013-11-27- 27"; volume: "5"; issue: "4"; pages: "878-85"
COMMENT     Sequence Label: YFP-Parkin-IRES-zeo
FEATURES             Location/Qualifiers
     source          1..14460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..470
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    535..892
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             957..1373
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1303..1325
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1341..1357
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1383..1757
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1695..1711
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             join(1781..1783,1784..1786,1787..2497)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1826..1847)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2087..2106)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2434..2455
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(3985..4559,4560..4562,4563..4571)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4152..4169)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4379..4398
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4572..4946
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     LTR             5169..5638
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             5903..6094
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(6088..6110)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6196..6336
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6251..6270
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6351..6368)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6522..7110)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6602..6621)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    7233..7367
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7257..7276)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7311..7330
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7597..8196)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7680..7700)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8177..8196
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(8279..8778)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8327..8346)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     8720..8743
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(join(12959..13750,13751..13819))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13582..13601
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13820..13924)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
       61 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
      121 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      181 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      241 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      301 aaaagagccc acaacccctc actcggcgcg ccagtcctcc gattgactga gtcgcccggg
      361 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      421 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
      481 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg
      541 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
      601 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
      661 gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg
      721 tggcccgacc tgagtccaaa aatcccgatc gttttggact ctttggtgca ccccccttag
      781 aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
      841 aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg
      901 ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaatatg ggcccgggcc
      961 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
     1021 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
     1081 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
     1141 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
     1201 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
     1261 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
     1321 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gcccccatat
     1381 ggccatatga gatcttatat ggggcacccc cgccccttgt aaacttccct gaccctgaca
     1441 tgacaagagt tactaacagc ccctctctcc aagctcactt acaggctctc tacttagtcc
     1501 agcacgaagt ctggagacct ctggcggcag cctaccaaga acaactggac cgaccggtgg
     1561 tacctcaccc ttaccgagtc ggcgacacag tgtgggtccg ccgacaccag actaagaacc
     1621 tagaacctcg ctggaaagga ccttacacag tcctgctgac cacccccacc gccctcaaag
     1681 tagacggcat cgcagcttgg atacacgccg cccacgtgaa ggctgccgac cccgggggtg
     1741 gaccatcctc tagactgccg gatctaagta agtaggatcc atggtgagca agggcgagga
     1801 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     1861 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     1921 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta
     1981 cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     2041 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     2101 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     2161 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     2221 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     2281 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     2341 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc
     2401 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     2461 cgccgggatc actctcggca tggacgagct gtacaagtcc ggactcagat ctcgagctca
     2521 agctttgata gtgtttgtca ggttcaactc cagccatggt ttcccagtgg aggtcgattc
     2581 tgacaccagc atcttccagc tcaaggaggt ggttgctaag cgacaggggg ttccggctga
     2641 ccagttgcgt gtgattttcg cagggaagga gctgaggaat gactggactg tgcagaattg
     2701 tgacctggat cagcagagca ttgttcacat tgtgcagaga ccgtggagaa aaggtcaaga
     2761 aatgaatgca actggaggcg acgaccccag aaacgcggcg ggaggctgtg agcgggagcc
     2821 ccagagcttg actcgggtgg acctcagcag ctcagtcctc ccaggagact ctgtggggct
     2881 ggctgtcatt ctgcacactg acagcaggaa ggactcacca ccagctggaa gtccagcagg
     2941 tagatcaatc tacaacagct tttatgtgta ttgcaaaggc ccctgtcaaa gagtgcagcc
     3001 gggaaaactc agggtacagt gcagcacctg caggcaggca acgctcacct tgacccaggg
     3061 tccatcttgc tgggatgatg ttttaattcc aaaccggatg agtggtgaat gccaatcccc
     3121 acactgccct gggactagtg cagaattttt ctttaaatgt ggagcacacc ccacctctga
     3181 caaggaaaca tcagtagctt tgcacctgat cgcaacaaat agtcggaaca tcacttgcat
     3241 tacgtgcaca gacgtcagga gccccgtcct ggttttccag tgcaactccc gccacgtgat
     3301 ttgcttagac tgtttccact tatactgtgt gacaagactc aatgatcggc agtttgtcca
     3361 cgaccctcag cttggctact ccctgccttg tgtggctggc tgtcccaact ccttgattaa
     3421 agagctccat cacttcagga ttctgggaga agagcagtac aaccggtacc agcagtatgg
     3481 tgcagaggag tgtgtcctgc agatgggggg cgtgttatgc ccccgccctg gctgtggagc
     3541 ggggctgctg ccggagcctg accagaggaa agtcacctgc gaagggggca atggcctggg
     3601 ctgtgggttt gccttctgcc gggaatgtaa agaagcgtac catgaagggg agtgcagtgc
     3661 cgtatttgaa gcctcaggaa caactactca ggcctacaga gtcgatgaaa gagccgccga
     3721 gcaggctcgt tgggaagcag cctccaaaga aaccatcaag aaaaccacca agccctgtcc
     3781 ccgctgccat gtaccagtgg aaaaaaatgg aggctgcatg cacatgaagt gtccgcagcc
     3841 ccagtgcagg ctcgagtggt gctggaactg tggctgcgag tggaaccgcg tctgcatggg
     3901 ggaccactgg ttcgacgtgt aggaattcct gcaggcctcg agggccggcg cgccgcggcc
     3961 gctacgtaaa ttcnnnnccc cccccccctc tccctccccc scccctaacg ttactggccg
     4021 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     4081 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     4141 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     4201 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     4261 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     4321 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     4381 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     4441 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
     4501 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
     4561 tggccacaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
     4621 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
     4681 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
     4741 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
     4801 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
     4861 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
     4921 acttcgtggc cgaggagcag gactgaccga cgccgaccaa caccgccggt ccgacgcggc
     4981 ccgacgggtc cgaggggggt cgacgataaa ataaaagatt ttatttagtc tccagaaaaa
     5041 ggggggaatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg
     5101 caaggcatgg aaaaatacat aactgagaat agagaagttc agatcaaggt caggaacaga
     5161 tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
     5221 agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
     5281 ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca
     5341 gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc
     5401 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg
     5461 agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt
     5521 cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg
     5581 ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcaca
     5641 tgcagcatgt atcaaaatta gcatcgcagg atgctgctgg ctaccctgtg gaacacctac
     5701 atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat
     5761 ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt
     5821 aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa
     5881 tcccccttac acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg
     5941 ctttatcaga agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga
     6001 acaggcagac atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct
     6061 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     6121 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     6181 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
     6241 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     6301 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
     6361 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     6421 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     6481 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     6541 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     6601 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     6661 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     6721 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     6781 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     6841 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     6901 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     6961 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     7021 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     7081 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     7141 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     7201 atcttcacct agatcctttt gcggccgaat tagatccaga catgataaga tacattgatg
     7261 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     7321 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     7381 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     7441 acctctacaa atgtggtatg gctgattatg atcctctaga gtcggtgggc ctcgggggcg
     7501 ggtgcggggt cggcggggcc gccccgggtg gcttcggtcg gagccatggg gtcgtgcgct
     7561 cctttcggtc gggcgctgcg ggtcgtgggg cgggcgtcag gcaccgggct tgcgggtcat
     7621 gcaccaggtg cgcggtcctt cgggcacctc gacgtcggcg gtgacggtga agccgagccg
     7681 ctcgtagaag gggaggttgc ggggcgcgga ggtctccagg aaggcgggca ccccggcgcg
     7741 ctcggccgcc tccactccgg ggagcacgac ggcgctgccc agacccttgc cctggtggtc
     7801 gggcgagacg ccgacggtgg ccaggaacca cgcgggctcc ttgggccggt gcggcgccag
     7861 gaggccttcc atctgttgct gcgcggccag ccgggaaccg ctcaactcgg ccatgcgcgg
     7921 gccgatctcg gcgaacaccg cccccgcttc gacgctctcc ggcgtggtcc agaccgccac
     7981 cgcggcgccg tcgtccgcga cccacacctt gccgatgtcg agcccgacgc gcgtgaggaa
     8041 gagttcttgc agctcggtga cccgctcgat gtggcggtcc gggtcgacgg tgtggcgcgt
     8101 ggcggggtag tcggcgaacg cggcggcgag ggtgcgtacg gcccggggga cgtcgtcgcg
     8161 ggtggcgagg cgcaccgtgg gcttgtactc ggtcatggaa ggtcgtctcc ttgtgagggg
     8221 tcaggggcgt gggtcagggg atggtggcgg caccggtcgt ggcggccgac ctgcaggtcg
     8281 aaaggcccgg agatgaggaa gaggagaaca gcgcggcaga cgtgcgcttt tgaagcgtgc
     8341 agaatgccgg gcctccggag gaccttcggg cgcccgcccc gcccctgagc ccgcccctga
     8401 gcccgccccc ggacccaccc cttcccagcc tctgagccca gaaagcgaag gagcaaagct
     8461 gctattggcc gctgccccaa aggcctaccc gcttccattg ctcagcggtg ctgtccatct
     8521 gcacgagact agtgagacgt gctacttcca tttgtcacgt cctgcacgac gcgagctgcg
     8581 gggcgggggg gaacttcctg actaggggag gagtagaagg tggcgcgaag gggccaccaa
     8641 agaacggagc cggttggcgc ctaccggtgg atgtggaatg tgtgcgaggc cagaggccac
     8701 ttgtgtagcg ccaagtgccc agcggggctg ctaaagcgca tgctccagac tgccttggga
     8761 aaagcgcctc ccctacccgg tagaattaat tctcatgttt gacagcttat catcgataag
     8821 ctgatcctca caggccgcac ccagcttttc ttccgttgcc ccagtagcat ctctgtctgg
     8881 tgaccttgaa gaggaagagg aggggtcccg agaatcccca tccctaccgt ccagcaaaaa
     8941 gggggacgag gaatttgagg cctggcttga ggctcaggac gcaaatcttg aggatgttca
     9001 gcgggagttt tccgggctgc gagtaattgg tgatgaggac gaggatggtt cggaggatgg
     9061 ggaattttca gacctggatc tgtctgacag cgaccatgaa ggggatgagg gtgggggggc
     9121 tgttggaggg ggcaggagtc tgcactccct gtattcactg agcgtcgtct aataaagatg
     9181 tctattgatc tcttttagtg tgaatcatgt ctgacgaggg gccaggtaca ggacctggaa
     9241 atggcctagg agagaaggga gacacatctg gaccagaagg ctccggcggc agtggacctc
     9301 aaagaagagg gggtgataac catggacgag gacggggaag aggacgagga cgaggaggcg
     9361 gaagaccagg agccccgggc ggctcaggat cagggccaag acatagagat ggtgtccgga
     9421 gaccccaaaa acgtccaagt tgcattggct gcaaagggac ccacggtgga acaggagcag
     9481 gagcaggagc rggaggggca ggagcaggag gnggnncagg agcaggagga ggggcaggag
     9541 nnncaggagg gnngsaggag ncaggaggag gggcaggagc aggaggggca ggagcaggag
     9601 gtggaggccg gggtcgagga ggcagtggag gccggggtcg aggaggtagt ggaggccggg
     9661 gtcgaggagg tagtggaggc cgccggggta gaggacgtga aagagccagg gggggaagtc
     9721 gtgaaagagc cagggggaga ggtcgtggac gtggagaaaa gaggcccagg agtcccagta
     9781 gtcagtcatc atcatccggg tctccaccgc gcaggccccc tccaggtaga aggccatttt
     9841 tccaccctgt aggggaagcc gattattttg aataccacca agaaggtggc ccagatggtg
     9901 agcctgacgt gcccccggga gcgatagagc agggccccgc agatgaccca ggagaaggcc
     9961 caagcactgg accccggggt cagggtgatg gaggcaggcg caaaaaagga gggtggtttg
    10021 gaaagcatcg tggtcaagga ggttccaacc cgaaatttga gaacattgca gaaggtttaa
    10081 gagctctcct ggctaggagt cacgtagaaa ggactaccga cgaaggaact tgggtcgccg
    10141 gtgtgttcgt atatggaggt agtaagacct ccctttacaa cctaaggcga ggaactgccc
    10201 ttgctattcc acaatgtcgt cttacaccat tgagtcgtct cccctttgga atggcccctg
    10261 gacccggccc acaacctggc ccgctaaggg agtccattgt ctgttatttc atggtctttt
    10321 tacaaactca tatatttgct gaggttttga aggatgcgat taaggacctt gttatgacaa
    10381 agcccgctcc tacctgcaat atcagggtga ctgtgtgcag ctttgacgat ggagtagatt
    10441 tgcctccctg gtttccacct atggtggaag gggctgccgc ggagggtgat gacggagatg
    10501 acggagatga aggaggtgat ggagatgagg gtgaggaagg gcaggagtga tgtaacttgt
    10561 taggagacgc cctcaatcgt attaaaagcc gtgtattccc ccgcactaaa gaataaatcc
    10621 ccagtagaca tcatgcgtgc tgttggtgta tttctggcca tctgtcttgt caccattttc
    10681 gtcctcccaa catggggcaa ttgggcatac ccatgttgtc acgtcactca gctccgcgct
    10741 caacaccttc tcgcgttgga aaacattagc gacatttacc tggtgagcaa tcagacatgc
    10801 gacggcttta gcctggcctc cttaaattca cctaagaatg ggagcaacca gcaggaaaag
    10861 gacaagcagc gaaaattcac gcccccttgg gaggtggcgg catatgcaaa ggatagcact
    10921 cccactctac tactgggtat catatgctga ctgtatatgc atgaggatag catatgctac
    10981 ccggatacag attaggatag catatactac ccagatatag attaggatag catatgctac
    11041 ccagatatag attaggatag cctatgctac ccagatataa attaggatag catatactac
    11101 ccagatatag attaggatag catatgctac ccggatacag attaggatag catatactac
    11161 ccagatatag attaggatag catatgctac ccagatatag attaggatag cctatgctac
    11221 ccagatataa attaggatag catatactac ccagatatag attaggatag catatgctac
    11281 ccagatatag attaggatag cctatgctac ccagatatag attaggatag catatgctat
    11341 ccagatattt gggtagtata tgctacccat ggcaacatta gcccaccgtg ctctcagcga
    11401 cctcgtgaat atgaggacca acaaccctgt gcttggcgct caggcgcaag tgtgtgtaat
    11461 ttgtcctcca gatcgcagca atcgcgcccc tatcttggcc cgcccaccta cttatgcagg
    11521 tattccccgg ggtgccatta gtggttttgt gggcaagtgg tttgaccgca gtggttagcg
    11581 gggttacaat cagccaagtt attacaccct tattttacag tccaaaaccg cagggcggcg
    11641 tgtgggggct gacgcgtgcc cccactccac aatttcaaaa aaaagagtgg ccacttgtct
    11701 ttgtttatgg gccccattgg cgtggagccc cgtttaattt tcgggggtgt tagagacaac
    11761 cagtggagtc cgctgctgtc ggcgtccact ctctttcccc ttgttacaaa tagagtgtaa
    11821 caacatggtt cacctgtctt ggtccctgcc tgggacacat cttaataacc ccagtatcat
    11881 attgcactag gattatgtgt tgcccatagc cataaattcg tgtgagatgg acatccagtc
    11941 tttacggctt gtccccaccc catggatttc tattgttaaa gatattcaga atgtttcatt
    12001 cctacactag tatttattgc ccaaggggtt tgtgagggtt atattggtgt catagcacaa
    12061 tgccaccact gaaccccccg tccaaatttt attctggggg cgtcacctga aaccttgttt
    12121 tcgagcacct cacatacacc ttactgttca caactcagca gttattctat tagctaaacg
    12181 aaggagaatg aagaagcagg cgaagattca ggagagttca ctgcccgctc cttgatcttc
    12241 agccactgcc cttgtgacta aaatggttca ctaccctcgt ggaatcctga ccccatgtaa
    12301 ataaaaccgt gacagctcat ggggtgggag atatcgctgt tccttaggac ccttttacta
    12361 accctaattc gatagcatat gcttcccgtt gggtaacata tgctattgaa ttagggttag
    12421 tctggatagt atatactact acccgggaag catatgctac ccgtttaggg ttaacaaggg
    12481 ggccttataa acactattgc taatgccctc ttgagggtcc gcttatcggt agctacacag
    12541 gcccctctga ttgacgttgg tgtagcctcc cgtagtcttc ctgggcccct gggaggtaca
    12601 tgtcccccag cattggtgta agagcttcag ccaagagtta cacataaagg caatgttgtg
    12661 ttgcagtcca cagactgcaa agtctgctcc aggatgaaag ccactcagtg ttggcaaatg
    12721 tgcacatcca tttataagga tgtcaactac agtcagagaa cccctttgtg tttggtcccc
    12781 ccccgtgtca catgtggaac agggcccagt tggcaagttg taccaaccaa ctgaagggat
    12841 tacatgcact gccccgcgaa gaaggggcag agatgtcgta gtcaggttta gttcgtccgg
    12901 ggcggggatc tggccgcaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    12961 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    13021 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    13081 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    13141 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    13201 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    13261 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    13321 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    13381 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    13441 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    13501 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    13561 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    13621 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    13681 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    13741 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    13801 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    13861 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    13921 cgcgcacatt tccccgaaaa gtgccaccag ctttgctctt aggagtttcc taatacatcc
    13981 caaactcaaa tatataaagc atttgacttg ttctatgccc tagggggcgg ggggaagcta
    14041 agccagcttt ttttaacatt taaaatgtta attccatttt aaatgcacag atgtttttat
    14101 ttcataaggg tttcaatgtg catgaatgct gcaatattcc tgttaccaaa gctagtataa
    14161 ataaaaatag ataaacgtgg aaattactta gagtttctgt cattaacgtt tccttcctca
    14221 gttgacaaca taaatgcgct gctgagaagc cagtttgcat ctgtcaggat caatttccca
    14281 ttatgccagt catattaatt actagtcaat tagttgattt ttatttttga catatacatg
    14341 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
    14401 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca gatggaacag
//
