LOCUS       sequence-365724-        8644 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mouse HDAC7-S (1-938) in mammalian cells (V5 tag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8644)
  AUTHORS   Shakespear MR, Hohenhaus DM, Kelly GM, Kamal NA, Gupta P, Labzin LI,
            Schroder K, Garceau V, Barbero S, Iyer A, Hume DA, Reid RC, Irvine 
            KM, Fairlie DP, Sweet MJ
  TITLE     Histone deacetylase 7 promotes Toll-like receptor 4-dependent 
            proinflammatory gene expression in macrophages.
  JOURNAL   J Biol Chem. 2013 Aug 30;288(35):25362-74. doi: 
            10.1074/jbc.M113.496281. Epub 2013 Jul 12.
  PUBMED    23853092
REFERENCE   2  (bases 1 to 8644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M113.496281"; journalName: "J Biol Chem"; date: 
            "2013-08-30- 30"; volume: "288"; issue: "35"; pages: "25362-74"
COMMENT     Sequence Label: mHDAC7-Spliced (1-938) pEF6-V5 (MJS_014)  [Edit]
FEATURES             Location/Qualifiers
     source          1..8644
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(166..185)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        473..1651
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          704..1642
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1599..1619
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1668..1687
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1668..1686
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4642..4683
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4693..4710
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4733..4750)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4739..4963
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5009..5437
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5096..5115)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5306..5327
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5446..5466)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5451..5780
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5631..5766
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5693..5712
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5828..5875
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5894..6292
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    6450..6571
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6487..6506)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6541..6560
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6620..6636)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6620..6636)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6633..6655)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6644..6660
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6668..6674,6675..6692,6693..6698))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6713..6734
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6851..6868)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7011..7599)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7091..7110)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7770..8561,8562..8630))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8393..8412
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(8631..8644,1..91))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
       61 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
      121 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
      181 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      241 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      301 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      361 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga
      421 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg
      481 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
      541 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
      601 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
      661 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
      721 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
      781 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
      841 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
      901 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
      961 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
     1021 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
     1081 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
     1141 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
     1201 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
     1261 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
     1321 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
     1381 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
     1441 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
     1501 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
     1561 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
     1621 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac
     1681 tatagggaga cccaagctgg ctaggtaagc ttggtaccga gctcggatcc actagtccag
     1741 tgtggtggaa ttgcccttgg gatgcatagt cccggcgcgg gctgccctgc cctccagcca
     1801 gacacaccag gctctcagcc ccaacccatg gacctgcggg tgggccagcg gcccacggtg
     1861 gagcccccac cagagcctgc gctgctgacc ctgcaacacc cccaacgcct gcaccgccat
     1921 ctcttcctgg caggcttaca ccagcaacag cgctcagccg agcccatgag gctctccatg
     1981 gacccaccaa tgccggagct gcagggggga cagcaggagc aagaacttcg gcaacttctc
     2041 aataaagaca agagcaagcg aagtgccgta gccagcagtg tggtcaagca gaagctggct
     2101 gaagtgatcc tgaagaaaca gcaggcagcc cttgagagaa cagtccatcc cagcagcccc
     2161 agtattccct acagaactct tgagcccttg gacacagagg gtgctgcccg ctccgtgctt
     2221 agcagcttcc tgcctcctgt tcccagcctg cccactgaac ccccggaaca ctttcccttg
     2281 cgtaaaacag tgtctgaacc caacctgaag ttgcgctaca aacccaagaa atccctggag
     2341 agacgcaaga atcccctgct caggaaggag agtgccccgc ccagccttcg gaggaggcct
     2401 gccgagaccc ttggagattc ctcccccagt agtagcagca cacccgcgtc agggtgcagc
     2461 tcccctaatg acagcgagca tggccctaac cctgccctag gctcagaggc tgatggtgac
     2521 cgcaggaccc attcaacttt aggccctcgg ggtcctgtac tggggaaccc ccatgctccc
     2581 ctcttcctgc accacggtct ggagccagag gctgggggca ccttaccctc tcgcctgcaa
     2641 cccattctcc tgctggaccc ctcagtctct catgccccac tgtggactgt gcctggcctt
     2701 gggcccttgc ccttccactt tgcccagccc ttactgacca ccgagcggct ctctgggtca
     2761 ggcctccatc gaccacttaa ccggacccgc tcagagcccc tgccccccag cgccacagcc
     2821 tcccctctgc tggcccccct gcagccccgc caggatcggc tcaaacctca cgtccagctg
     2881 atcaagccag ccatctcccc tccccagagg cctgccaagc ccagtgagaa gccccgactg
     2941 cgacagatac cctcggctga ggacctagag acagatggtg ggggagtggg acctatggcg
     3001 aatgatggcc tggaacatag ggagtcaggc cgtgggcctc ctgagggcag aggctccatt
     3061 tctctgcagc agcaccaaca ggtgccaccc tgggagcagc agcatctagc cgggcggctc
     3121 tctcagggaa gccccgggga ctccgtgctg atacctctgg cccaggttgg acaccggccc
     3181 ctgtccagaa cccagtcttc cccagcagca cctgtctcca tgctgagccc agagcccacc
     3241 tgtcagaccc aagtcctcaa cagctcagag acacctgcta cagggctggt ctatgactcg
     3301 gtgatgctga aacaccaatg ttcctgtgga gacaacagca agcatcccga gcatgcaggc
     3361 cgcatccaga gcatctggtc ccggctgcag gaacggggtc tccgcagcca gtgtgagtgt
     3421 ctccgaggcc gaaaggcttc cctagaggag ctgcagtcag tccactctga acggcacgtg
     3481 ctcctctacg gcacgaaccc actcagccgc ctcaaactgg ataacgggaa gcttacaggg
     3541 ctcctggcac agcggacgtt tgtgatgcta ccctgtggcg gggttggggt cgatactgac
     3601 accatctgga acgagctgca ttcctccaat gcagcccgct gggctgcggg cagcgtcacc
     3661 gaccttgcct tcaaagtagc ttcccgagag ctgaagaacg gctttgctgt ggtgcgaccc
     3721 ccgggacacc atgcagatca ttctacagcc atgggcttct gcttcttcaa ctccgtggcc
     3781 atcgcctgcc gacagctaca gcaacacggc aaagccagca agatcctcat tgttgactgg
     3841 gatgttcacc atggcaacgg cacacagcag actttctacc aggaccccag tgtgctctac
     3901 atttcccttc atcgtcatga cgacggcaac ttcttcccag gcagtggggc cgtggatgag
     3961 gtgggaactg gcagtggcga gggcttcaat gtcaacgtgg cttgggctgg gggcttggat
     4021 ccacccatgg gggatcctga gtacctggct gctttcagga tagtggtgat gcccattgcc
     4081 cgagagtttg ctccagacct ggtcctggtg tctgctgggt ttgatgctgc ggagggtcac
     4141 ccagccccac tgggtggcta ccatgtttct gccaaatgtt ttgggtacat gacgcagcag
     4201 ttgatgaact tggcaggagg cgccgtggtg ttggccttag agggtggaca tgacctcacg
     4261 gccatctgtg atgcctcgga ggcctgtgta gctgctcttc tgggcaacaa ggtggacccc
     4321 ctttcagaag aaagctggaa acagaaaccc aacctcagtg ccatccgctc gctggaagct
     4381 gtggtcaggg tgcacaggaa atactggggc tgcatgcagc gcttggcctc ctgtccagac
     4441 tcctggctac ccagagtgcc gggagctgat gcagaagtgg aagccgtgac cgcgctggca
     4501 tccctttctg tgggcatcct ggctgaagac aggccctcgg agcggctggt ggaagaggaa
     4561 gaacccatga acctcaaggg caattctgca gatatccagc acagtggcgg ccgctcgagt
     4621 ctagagggcc cgcggttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct
     4681 acgcgtaccg gtcatcatca ccatcaccat tgagtttaaa cccgctgatc agcctcgact
     4741 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     4801 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     4861 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     4921 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     4981 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     5041 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     5101 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     5161 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     5221 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     5281 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     5341 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     5401 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     5461 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     5521 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     5581 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     5641 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     5701 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     5761 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     5821 agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt
     5881 gaggaactaa accatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc
     5941 aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct
     6001 ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg
     6061 tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat
     6121 cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt
     6181 gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac
     6241 ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct aagcacttcg
     6301 tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg
     6361 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     6421 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     6481 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6541 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     6601 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     6661 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     6721 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     6781 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     6841 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     6901 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     6961 acatgtgagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7021 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7081 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7141 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7201 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7261 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7321 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7381 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7441 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7501 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7561 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7621 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7681 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7741 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7801 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7861 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7921 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     7981 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8041 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8101 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8161 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8221 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8281 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8341 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8401 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8461 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8521 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8581 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8641 tttc
//
