LOCUS       sequence-365742-        8587 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  [Edit] Expression of mouse HDAC7-S (1-938) in mammalian cells (Flag 
            tag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8587)
  AUTHORS   Shakespear MR, Hohenhaus DM, Kelly GM, Kamal NA, Gupta P, Labzin LI,
            Schroder K, Garceau V, Barbero S, Iyer A, Hume DA, Reid RC, Irvine 
            KM, Fairlie DP, Sweet MJ
  TITLE     Histone deacetylase 7 promotes Toll-like receptor 4-dependent 
            proinflammatory gene expression in macrophages.
  JOURNAL   J Biol Chem. 2013 Aug 30;288(35):25362-74. doi: 
            10.1074/jbc.M113.496281. Epub 2013 Jul 12.
  PUBMED    23853092
REFERENCE   2  (bases 1 to 8587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M113.496281"; journalName: "J Biol Chem"; date: 
            "2013-08-30- 30"; volume: "288"; issue: "35"; pages: "25362-74"
COMMENT     Sequence Label: mHDAC7-Spliced (1-938) pEF6-Flag (MJS_015)
FEATURES             Location/Qualifiers
     source          1..8587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2869..2892
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(2915..2932)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2921..3145
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3191..3619
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3278..3297)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3488..3509
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3628..3648)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3633..3962
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3813..3948
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3875..3894
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4010..4057
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4076..4474
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    4632..4753
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4669..4688)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4723..4742
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4802..4818)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4802..4818)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4815..4837)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4826..4842
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4850..4856,4857..4874,4875..4880))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4895..4916
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5033..5050)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5193..5781)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5273..5292)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5952..6743,6744..6812))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6575..6594
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6813..6917)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6992..7011)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7299..8477
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7530..8468
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8425..8445
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     8494..8513
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8494..8512
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atgcatagtc ccggcgcggg ctgccctgcc ctccagccag acacaccagg ctctcagccc
       61 caacccatgg acctgcgggt gggccagcgg cccacggtgg agcccccacc agagcctgcg
      121 ctgctgaccc tgcaacaccc ccaacgcctg caccgccatc tcttcctggc aggcttacac
      181 cagcaacagc gctcagccga gcccatgagg ctctccatgg acccaccaat gccggagctg
      241 caggggggac agcaggagca agaacttcgg caacttctca ataaagacaa gagcaagcga
      301 agtgccgtag ccagcagtgt ggtcaagcag aagctggctg aagtgatcct gaagaaacag
      361 caggcagccc ttgagagaac agtccatccc agcagcccca gtattcccta cagaactctt
      421 gagcccttgg acacagaggg tgctgcccgc tccgtgctta gcagcttcct gcctcctgtt
      481 cccagcctgc ccactgaacc cccggaacac tttcccttgc gtaaaacagt gtctgaaccc
      541 aacctgaagt tgcgctacaa acccaagaaa tccctggaga gacgcaagaa tcccctgctc
      601 aggaaggaga gtgccccgcc cagccttcgg aggaggcctg ccgagaccct tggagattcc
      661 tcccccagta gtagcagcac acccgcgtca gggtgcagct cccctaatga cagcgagcat
      721 ggccctaacc ctgccctagg ctcagaggct gatggtgacc gcaggaccca ttcaacttta
      781 ggccctcggg gtcctgtact ggggaacccc catgctcccc tcttcctgca ccacggtctg
      841 gagccagagg ctgggggcac cttaccctct cgcctgcaac ccattctcct gctggacccc
      901 tcagtctctc atgccccact gtggactgtg cctggccttg ggcccttgcc cttccacttt
      961 gcccagccct tactgaccac cgagcggctc tctgggtcag gcctccatcg accacttaac
     1021 cggacccgct cagagcccct gccccccagc gccacagcct cccctctgct ggcccccctg
     1081 cagccccgcc aggatcggct caaacctcac gtccagctga tcaagccagc catctcccct
     1141 ccccagaggc ctgccaagcc cagtgagaag ccccgactgc gacagatacc ctcggctgag
     1201 gacctagaga cagatggtgg gggagtggga cctatggcga atgatggcct ggaacatagg
     1261 gagtcaggcc gtgggcctcc tgagggcaga ggctccattt ctctgcagca gcaccaacag
     1321 gtgccaccct gggagcagca gcatctagcc gggcggctct ctcagggaag ccccggggac
     1381 tccgtgctga tacctctggc ccaggttgga caccggcccc tgtccagaac ccagtcttcc
     1441 ccagcagcac ctgtctccat gctgagccca gagcccacct gtcagaccca agtcctcaac
     1501 agctcagaga cacctgctac agggctggtc tatgactcgg tgatgctgaa acaccaatgt
     1561 tcctgtggag acaacagcaa gcatcccgag catgcaggcc gcatccagag catctggtcc
     1621 cggctgcagg aacggggtct ccgcagccag tgtgagtgtc tccgaggccg aaaggcttcc
     1681 ctagaggagc tgcagtcagt ccactctgaa cggcacgtgc tcctctacgg cacgaaccca
     1741 ctcagccgcc tcaaactgga taacgggaag cttacagggc tcctggcaca gcggacgttt
     1801 gtgatgctac cctgtggcgg ggttggggtc gatactgaca ccatctggaa cgagctgcat
     1861 tcctccaatg cagcccgctg ggctgcgggc agcgtcaccg accttgcctt caaagtagct
     1921 tcccgagagc tgaagaacgg ctttgctgtg gtgcgacccc cgggacacca tgcagatcat
     1981 tctacagcca tgggcttctg cttcttcaac tccgtggcca tcgcctgccg acagctacag
     2041 caacacggca aagccagcaa gatcctcatt gttgactggg atgttcacca tggcaacggc
     2101 acacagcaga ctttctacca ggaccccagt gtgctctaca tttcccttca tcgtcatgac
     2161 gacggcaact tcttcccagg cagtggggcc gtggatgagg tgggaactgg cagtggcgag
     2221 ggcttcaatg tcaacgtggc ttgggctggg ggcttggatc cacccatggg ggatcctgag
     2281 tacctggctg ctttcaggat agtggtgatg cccattgccc gagagtttgc tccagacctg
     2341 gtcctggtgt ctgctgggtt tgatgctgcg gagggtcacc cagccccact gggtggctac
     2401 catgtttctg ccaaatgttt tgggtacatg acgcagcagt tgatgaactt ggcaggaggc
     2461 gccgtggtgt tggccttaga gggtggacat gacctcacgg ccatctgtga tgcctcggag
     2521 gcctgtgtag ctgctcttct gggcaacaag gtggaccccc tttcagaaga aagctggaaa
     2581 cagaaaccca acctcagtgc catccgctcg ctggaagctg tggtcagggt gcacaggaaa
     2641 tactggggct gcatgcagcg cttggcctcc tgtccagact cctggctacc cagagtgccg
     2701 ggagctgatg cagaagtgga agccgtgacc gcgctggcat ccctttctgt gggcatcctg
     2761 gctgaagaca ggccctcgga gcggctggtg gaagaggaag aacccatgaa cctcaagggc
     2821 aattctgcag atatccagca cagtggcggc cgctcgagtc tagagggcga ctacaaagac
     2881 gatgacgaca agtgagttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     2941 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     3001 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     3061 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     3121 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     3181 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     3241 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     3301 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     3361 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     3421 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     3481 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     3541 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     3601 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     3661 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     3721 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     3781 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     3841 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     3901 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     3961 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta
     4021 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc
     4081 caagcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag
     4141 catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat
     4201 cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct
     4261 gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga
     4321 gaacaggggc atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc
     4381 tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga
     4441 attgctgccc tctggttatg tgtgggaggg ctaagcactt cgtggccgag gagcaggact
     4501 gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     4561 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     4621 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     4681 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     4741 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     4801 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     4861 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     4921 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     4981 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     5041 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     5101 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     5161 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     5221 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     5281 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     5341 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     5401 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     5461 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     5521 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     5581 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     5641 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     5701 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     5761 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     5821 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     5881 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     5941 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     6001 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     6061 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     6121 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     6181 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     6241 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     6301 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     6361 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     6421 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     6481 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     6541 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     6601 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     6661 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     6721 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     6781 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     6841 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     6901 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
     6961 gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     7021 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
     7081 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
     7141 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta
     7201 ggcttttgca aaaagctttg caaagatgga taaagtttta aacagagagg aatctttgca
     7261 gctaatggac cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg
     7321 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     7381 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     7441 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     7501 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     7561 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     7621 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     7681 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     7741 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     7801 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     7861 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     7921 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     7981 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg
     8041 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     8101 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     8161 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     8221 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     8281 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     8341 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     8401 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     8461 catttcaggt gtcgtgagga attagcttgg tactaatacg actcactata gggagaccca
     8521 agctggctag gtaagcttgg taccgagctc ggatccacta gtccagtgtg gtggaattgc
     8581 ccttggg
//
