LOCUS       sequence-365745-        6738 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6738)
  AUTHORS   Xi Q, He W, Zhang XH, Le HV, Massague J
  TITLE     Genome-wide impact of the BRG1 SWI/SNF chromatin remodeler on the 
            transforming growth factor beta transcriptional program.
  JOURNAL   J Biol Chem. 2008 Jan 11. 283(2):1146-55.
  PUBMED    18003620
REFERENCE   2  (bases 1 to 6738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2008 Jan 11. 283(2):1146-55."
COMMENT     Sequence Label: pMYC BRGI aa597-1407
FEATURES             Location/Qualifiers
     source          1..6738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        92..1076
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        1111..1129
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1111..1128
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             1179..1208
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1218..1247
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1257..1286
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1296..1325
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1335..1364
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    3866..4000
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3890..3909)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3944..3963
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4113..4133)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4113..4131)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4152..4168)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4152..4168)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4165..4187)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4176..4192
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4200..4206,4207..4224,4225..4230))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4245..4266
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4383..4400)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4554..5142)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4634..4653)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5313..6104,6105..6173))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5936..5955
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6174..6278)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(join(6304..6738,1..21))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6441..6462)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6653..6672
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atgcgccgct acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc
       61 gatcggtgcg ggcctcttcg ctattacgcc agtcgaccgc caattcaata tggcgtatat
      121 ggactcatgc caattcaata tggtggatct ggacctgtgc caattcaata tggcgtatat
      181 ggactcgtgc caattcaata tggtggatct ggaccccagc caattcaata tggcggactt
      241 ggcaccatgc caattcaata tggcggacct ggcactgtgc caactgggga ggggtctact
      301 tggcacggtg ccaagtttga ggaggggtct tggccctgtg ccaagtccgc catattgaat
      361 tggcatggtg ccaataatgg cggccatatt ggctatatgc caggatcaat atataggcaa
      421 tatccaatat ggccctatgc caatatggct attggccagg ttcaatacta tgtattggcc
      481 ctatgccata tagtattcca tatatgggtt ttcctattga cgtagatagc ccctcccaat
      541 gggcggtccc atataccata tatggggctt cctaataccg cccatagcca ctcccccatt
      601 gacgtcaatg gtctctatat atggtctttc ctattgacgt catatgggcg gtcctattga
      661 cgtatatggc gcctccccca ttgacgtcaa ttacggtaaa tggcccgcct ggctcaatgc
      721 ccattgacgt caataggacc acccaccatt gacgtcaatg ggatggctca ttgcccattc
      781 atatccgttc tcacgccccc tattgacgtc aatgacggta aatggcccac ttggcagtac
      841 atcaatatct attaatagta acttggcaag tacattacta ttggaagtac gccagggtac
      901 attggcagta ctcccattga cgtcaatggc ggtaaatggc ccgcgatggc tgccaagtac
      961 atccccattg acgtcaatgg ggaggggcaa tgacgcaaat gggcgttcca ttgacgtaaa
     1021 tgggcggtag gcgtgcctaa tgggaggtct atataagcaa tgctcgttta gggaaccgcc
     1081 attctgcctg gggacgtcgg agcaagcttg atttaggtga cactatagaa tacaagctac
     1141 ttgttctttt tgcaggatcc catcgattta aagctatgga gcaaaagctc atttctgaag
     1201 aggacttgaa tgaaatggag caaaagctca tttctgaaga ggacttgaat gaaatggagc
     1261 aaaagctcat ttctgaagag gacttgaatg aaatggagca aaagctcatt tctgaagagg
     1321 acttgaatga aatggagcaa aagctcattt ctgaagagga cttgaatgaa atggagagct
     1381 tgggcgacct caccatggcg cctgccattg ggccggatgg cgagcctctg gacgagacca
     1441 gccagatgag cgacctcccg gtgaaggtga tccacgtgga gagtgggaag atcctcacag
     1501 gcacagatgc ccccaaagcc gggcagctgg aggcctggct cgagatgaac ccggggtatg
     1561 aagtagctcc gaggtctgat agtgaagaaa gtggctcaga agaagaggaa gaggaggagg
     1621 aggaagagca gccgcaggca gcacagcctc ccaccctgcc cgtggaggag aagaagaaga
     1681 ttccagatcc agacagcgat gacgtctctg aggtggacgc gcggcacatc attgagaatg
     1741 ccaagcaaga tgtcgatgat gaatatggcg tgtcccaggc ccttgcacgt ggcctgcagt
     1801 cctactatgc cgtggcccat gctgtcactg agagagtgga caagcagtca gcgcttatgg
     1861 tcaatggtgt cctcaaacag taccagatca aaggtttgga gtggctggtg tccctgtaca
     1921 acaacaacct gaacggcatc ctggccgacg agatgggcct ggggaagacc atccagacca
     1981 tcgcgctcat cacgtacctc atggagcaca aacgcatcaa tgggcccttc ctcatcatcg
     2041 tgcctctctc aacgctgtcc aactgggcgt acgagtttga caagtgggcc ccctccgtgg
     2101 tgaaggtgtc ttacaaggga tccccagcag caagacgggc ctttgtcccc cagctccgga
     2161 gtgggaagtt caacgtcttg ctgacgacgt acgagtacat catcaaagac aagcacatcc
     2221 tcgccaagat ccgttggaag tacatgattg tggacgaagg tcaccgcatg aagaaccacc
     2281 actgcaagct gacgcaggtg ctcaacacgc actatgtggc accccgccgc ctgctgctga
     2341 cgggcacacc gctgcagaac aagcttcccg agctctgggc gctgctcaac ttcctgctgc
     2401 ccaccatctt caagagctgc agcaccttcg agcagtggtt taacgcaccc tttgccatga
     2461 ccggggaaaa ggtggacctg aatgaggagg aaaccattct catcatccgg cgtctccaca
     2521 aagtgctgcg gcccttcttg ctccgacgac tcaagaagga agtcgaggcc cagttgcccg
     2581 aaaaggtgga gtacgtcatc aagtgcgaca tgtctgcgct gcagcgagtg ctctaccgcc
     2641 acatgcaggc caagggcgtg ctgctgactg atggctccga gaaggacaag aagggcaaag
     2701 gcggcaccaa gaccctgatg aacaccatca tgcagctgcg gaagatctgc aaccacccct
     2761 acatgttcca gcacatcgag gagtcctttt ccgagcactt ggggttcact ggcggcattg
     2821 tccaagggct ggacctgtac cgagcctcgg gtaaatttga gcttcttgat agaattcttc
     2881 ccaaactccg agcaaccaac cacaaagtgc tgctgttctg ccaaatgacc tccctcatga
     2941 ccatcatgga agattacttt gcgtatcgcg gctttaaata cctcaggctt gatggaacca
     3001 cgaaggcgga ggaccggggc atgctgctga aaaccttcaa cgagcccggc tctgagtact
     3061 tcatcttcct gctcagcacc cgggctgggg ggctcggcct gaacctccag tcggcagaca
     3121 ctgtgatcat ttttgacagc gactggaatc ctcaccagga cctgcaagcg caggaccgag
     3181 cccaccgcat cgggcagcag aacgaggtgc gtgtgctccg cctctgcacc gtcaacagcg
     3241 tggaggagaa gatcctagct gcagccaagt acaagctcaa cgtggaccag aaggtgatcc
     3301 aggccggcat gttcgaccag aagtcctcca gccatgagcg gcgcgccttc ctgcaggcca
     3361 tcctggagca cgaggagcag gatgagagca gacactgcag cacgggcagc ggcagtgcca
     3421 gcttcgccca cactgcccct ccgccagcgg gcgtcaaccc cgacttggag gagccacctc
     3481 taaaggagga agacgaggtg cccgacgacg agaccgtcaa ccagatgatc gcccggcacg
     3541 aggaggagtt tgatctgttc atgcgcatgg acctggaccg caggcgcgag gaggcccgca
     3601 accccaagcg gaagccgcgc ctcatggagg aggacgagct cccctcgtgg atcatcaagg
     3661 acgacgcgga ggtggagcgg ctgacctgtg aggaggagga ggagaagatg ttcggccgtg
     3721 gctcccgcca ccgcaaggag gtggactaca gcgactcact gacggagaag cagtggctca
     3781 aggccatcga ggagggcacg ctggaggaga tcgaagagga ggtccggcag aagtaatcta
     3841 gaactatagt gagtcgtatt acgtagatcc agacatgata agatacattg atgagtttgg
     3901 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     3961 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     4021 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taattcgcgg ccgcggcgcc
     4081 aatgcattgg gcccggtacc cagcttttgt tccctttagt gagggttaat tgcgcgcttg
     4141 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     4201 aacatacgag ccgggagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     4261 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     4321 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     4381 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     4441 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     4501 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     4561 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     4621 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     4681 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     4741 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     4801 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     4861 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     4921 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     4981 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     5041 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     5101 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     5161 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     5221 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     5281 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     5341 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     5401 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     5461 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     5521 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     5581 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     5641 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     5701 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     5761 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     5821 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     5881 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     5941 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     6001 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     6061 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     6121 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     6181 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     6241 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     6301 cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     6361 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     6421 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     6481 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     6541 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     6601 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     6661 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     6721 ccacacccgc cgcgctta
//
