LOCUS       sequence-365764-        4404 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC6A2_STOP
FEATURES             Location/Qualifiers
     source          1..4404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1961..2060)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2077..2096)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2078..2096)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2101..2117)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2101..2117)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2219..2238)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2230..3039
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2297..2316)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3186..3774
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3675..3694
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3928..3945
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4104..4131)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4223..4309)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4358..4380
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4372..4389
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4373..4389
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgct gctggccagg
      121 atgaaccctc aggtgcagcc agagaacaat ggagcagaca ccggaccaga gcagccactg
      181 cgggccagaa agacagccga gctgctggtg gtgaaggaga ggaatggagt gcagtgcctg
      241 ctggcaccaa gggacggcga tgcacagcca agagagacct ggggcaagaa gatcgacttt
      301 ctgctgagcg tggtgggctt cgccgtggat ctggccaacg tgtggcggtt tccctacctg
      361 tgctataaga atggcggcgg cgcctttctg atcccttaca ccctgttcct gatcatcgcc
      421 ggcatgccac tgttctacat ggagctggcc ctgggccagt ataacagaga gggcgccgcc
      481 acagtgtgga agatctgccc attctttaag ggcgtgggct acgccgtgat cctgatcgcc
      541 ctgtatgtgg gcttctacta taacgtgatc atcgcctggt ctctgtacta tctgttcagc
      601 tcctttaccc tgaatctgcc ctggaccgac tgcggccaca catggaacag ccccaattgt
      661 acagatccta agctgctgaa cggcagcgtg ctgggcaatc acaccaagta ctccaagtat
      721 aagttcacac ctgccgccga gttttatgag cggggcgtgc tgcacctgca cgagtctagc
      781 ggcatccacg atatcggcct gccacagtgg cagctgctgc tgtgcctgat ggtggtggtc
      841 atcgtgctgt acttttccct gtggaagggc gtgaagacct ctggcaaggt ggtgtggatc
      901 accgccacac tgccatattt cgtgctgttt gtgctgctgg tgcacggagt gacactgcca
      961 ggagcctcca acggcatcaa tgcctacctg cacatcgact tctataggct gaaggaggcc
     1021 accgtgtgga tcgatgccgc cacacagatc ttctttagcc tgggagcagg attcggcgtg
     1081 ctgatcgcct ttgcctccta caacaagttc gacaacaatt gctatcgcga tgccctgctg
     1141 acctcctcta tcaattgtat cacaagcttc gtgtccggct tcgccatctt tagcatcctg
     1201 ggctacatgg cccacgagca caaagtgaat atcgaggacg tggcaaccga gggagcagga
     1261 ctggtgttta tcctgtatcc tgaggccatc tccaccctgt ccggctctac attctgggcc
     1321 gtggtgttct ttgtgatgct gctggccctg ggactggata gctccatggg aggcatggag
     1381 gccgtgatca caggcctggc cgacgatttt caggtgctga agaggcaccg caagctgttc
     1441 acctttggcg tgaccttctc cacatttctg ctggccctgt tctgcatcac aaagggcggc
     1501 atctacgtgc tgaccctgct ggacacattt gccgccggca cctctatcct gttcgccgtg
     1561 ctgatggagg ccatcggcgt gtcttggttt tacggcgtgg accggttcag caacgatatc
     1621 cagcagatga tgggctttag gcccggcctg tattggcgcc tgtgctggaa gttcgtgtcc
     1681 cctgccttcc tgctgtttgt ggtggtggtg tctatcatca actttaagcc actgacctac
     1741 gacgattata tcttcccacc ttgggcaaat tgggtgggat ggggaatcgc cctgtctagc
     1801 atggtgctgg tgcccatcta cgtgatctat aagtttctgt ctacccaggg aagcctgtgg
     1861 gagaggctgg catacggcat cacacctgag aatgagcacc acctggtggc ccagcgggat
     1921 atcagacagt tccagctgca gcactggctg gccatctaag acccagcttt cttgtacaaa
     1981 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
     2041 caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg
     2101 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     2161 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
     2221 aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg
     2281 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
     2341 atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt
     2401 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg
     2461 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact
     2521 gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat
     2581 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt
     2641 ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt
     2701 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg
     2761 aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc
     2821 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga
     2881 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt
     2941 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat
     3001 aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg
     3061 taacactggc agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc
     3121 cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3181 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3241 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3301 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     3361 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3421 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3481 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3541 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3601 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3661 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3721 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3781 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3841 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3901 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     3961 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     4021 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
     4081 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
     4141 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
     4201 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     4261 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     4321 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     4381 acggccagtc ttaagctcgg gccc
//
